Protective Effect Of PNS On Lower Limb Ischemia-reperfusion Injury In Rats

Objective:To observe the protective effect of PNS on the lower limb ischemia-reperfusion, especially the influence of PNS on SOD,MDA in serum and ultrastructure changes of skeletal muscle. Methods:Ventral median abdominal incision, the model of lower limb ischemia-reperfusion in rats was made by clamping aorta. Rats were randomly divided into five groups:sham-operated group, ischemic 1h group, ischemic 2h group, ischemic 1h+PNS treated group, ischemic 2h+PNS treated group. The levels of MDA and SOD in serum were checked by spectrophotometry, and ultrastructure changes of skeletal muscle were observed by transmission electron microscope. Results:The level of MDA in ischemic 1h group when reperfusion increased significantly when compared with sham-operated group, but the decrease of SOD was not obvious (P>0.05). The ultra-structure change of skeletal muscle was not notable and the damage recovered after one week. However, less myofilaments become fuzzy,mitochondrion swelling were observed in ischemic 1h+PNS treated group, after reperfusion 2h. The chang was lighter than ischemic 1h reperfusion 2h. Furthermore, the level of MDA increased and SOD decreased significantly in Ischemic 2h group (P<0.05). Local myofilament fracture and mitochondrion swelling can be checked in ischemic 2h after reperfusion 2h group. It is excitable that muscle segment was very clear, and the damage of mitochondrion was light in ischemic 2h+ PNS treated group, after reperfusion 2h. In ischemic 2h after reperfusion 2h group, the irreversible alteration such as pinosome, vessel wall necrosis blinding lumens have been seen. Ischemic 2hour+PNS treated group after reperfusion 2hour chromatin margination of the endothelial cell, red blood cell and granulocytes can be seen in lumens, basement membrane was difference. Conclusion:Lower limb injury was lighter when abdominal aorta block 1h and can recover. However, when the abdominal aorta block exceed 1h, the oxidative stress was severity and irreversible. The protective effect of PNS on Lower limb ischemia-reperfusion injury in rats was conspicuous.