The Level Of Microparticles Originated From Endothelial Cells And Its Significance In Diabetic Angiopathy

Microparticles (MPs) are small, membrane-derived vesicles, released by cells on activation or during apoptosis. These microparticles can provide valuable information on the status of the parent cells by expressing specific antigens. Recently, it had been reported that increased levels of platelet microparticles (PMPs),endothelial microparticlese (EMPs)or TF-bearing microparticles (TF+MPs) occur in association with several thrombosis diseases. We evaluated the levels of EMPs, PMPs and TF+MPs using flow cytometry in patients with type 2 diabetes mellitus (T2DM) and investigate the correlation between the concentrations of microparticles and diabetes complication.Objective To establish the method of absolute count of microparticles. To analyse the levels of EMPs, PMPs and TF+MPs in T2DM, and evaluate their relationship between the concentrations of microparticles and diabetes complications. Methods P-selectin positive rates on platelet surface was analysed in 126 patients with T2DM and 50 age-matched healthy volunteers served as the control. To detect TXB2 concentrations in plasma by an enzyme-linked immunosorbent assay (ELISA) kit;To establish the method of absolute count of PMPs,EMPs or TF+MPs, To measure the levels of EMPs,,PMPs and TF+MPs in control group and diabetes mellitus group by using flow cytometer, and to explore the differences of EMPs between groups of diabetic macroangiopathy and diabetic microangiopathy. Results PGE1 (3.0×10-7 M) can effectively inhibit platelet activation. The level of EMPs in diabetic macroangiopathy and diabetic microangiopathy were higher than that in T2DM alone, and they were markedly increased than those in control group, The concentrations of PMPs and TF+MPs has no distinct increase. Conclusion The Inhibition effect of PGE1 on platelet activation was dose dependent. Due to endothelial dysfunction, the level of EMPs was significantly elevated in type 2 diabetic especially in diabetic angiopathy which reflected endothelial function and status and the development of diabetic angiopathy.