The Significance And Expression Of DC-LAMP And DC-SIGN On Dendritic Cells In Psoriasis Vulgaris Lesions

Research Background Psoriasis is a common chronic inflammatory skin disease characterized by abnormal keratinocyte proliferation and differentiation, superficial dermal inflammatory cell infiltration and vascular proliferation as the basic pathology. At the present,the study of it's pathogenesis is mainly in immune disorders, abnormal epidermal keratinocyte proliferation and abnormal capillary proliferation, etc. More consistent view is that psoriasis is a multi-genetic background of immune-mediated inflammatory skin disease, and the existence of immune disorders is closely related to the psoriasis occur, development and recurrence. The abnormal activation of T lymphocytes in psoriasis lesions is the key to the disease. Meanwhile, dendritic cells, keratinocytes and other immune cells with their cytokines constitute the immune pathogenesis of psoriasis together.Dendritic cells possess all the features to activate naive T lymphocytes. Immature dendritic cells have strong antigen capture and processing functions, while Mature dendritic cells have strong antigen presenting functions.The relationship between Dendritic cells and T lymphocytes in the immune pathogenesis of psoriasis was attentioned by researchers. DC-lysosomal-associated membrane protein (DC-LAMP) is the marker of mature dendritic cells, belonging to lysosome associated membrane protein family. Its function is connected with processing MHC antigen complex and their intracellular transport in dendritic cells. Dendritic cell specific ICAM-3 grabbing non-integrin(DC-SIGN) is a newly discovered C-type lectin, expressed specificly in dendritic cells, regulating variety immune function of dendritic cells, playing an important role in Activated T lymphocytes. DC-LAMP and DC-SIGN are the key factors in regulation the relationship between dendritic cells and T lymphocytes.Research Objective In the study, We investigated the expression of DC-LAMP and DC-SIGN in normal skin and psoriasis vulgaris lesions, to explore the role of DC-LAMP and DC-SIGN in the pathogenic mechanism associated with psoriasis.Research Methods The expression and allocation of DC-LAMP and DC-SIGN were determined by immunohistochemistry and RT-PCR in 33 case psoriasis vulgaris paraffin imbedding samples, as well as in 11 case healthy individuals. Using image analysis technique, we analyzed the quantity of DC-LAMP and DC-SIGN expressed in psoriasis vulgaris and the normal skin. And their correlation analysis was performed.Results The PCR results showed that both psoriasis group and the normal group all amplify the goal fragment. The gene level expressed by psoriasis lesions were higher than the normal skins. DC-LAMP highly expressed in the cytoplasm of basal layer, spinous layer epidermis and dendritic cells in dermis. DC-SIGN highly expressed in the cytoplasm and the cytoblast of keratinocytes and dendritic cells in dermis. The expression of DC-LAMP and DC-SIGN in psoriasis vulgaris tissue were significant higher than that of normal skin, showing statistical significance (P<0.01). And in psoriasis lesions, the expression of DC-LAMP was positively correlated with the expression of DC-SIGN (P<0.05, r=0.368)Conclusionsâ‘ DC-LAMP located in the cytoplasm of epidermis keratinocyte and dendritic cells in dermis of psoriasis vulgaris lesions. Its expression of gene level and protein level were significant higher than that of normal skin.â‘¡DC-SIGN located in the cytoplasm and the cytoblast of keratinocytes and dendritic cells in dermis. Its expression of gene level and protein level were significant higher than that of normal skin.â‘¢The result of DC-LAMP was positively correlated with DC-SIGN in psoriasis shows DC-LAMP in coordination with DC-SIGN participating T lymphocytes.â‘£keratinocyte express DC-LAMP and DC-SIGN to active T lyphocytes deeply in psoriasis lesions.