Surface Modification Of Electrospun Poly(Lacic-co-glycolic Acid) Scaffold And Film With Hydrophobin And Their Application

Biopolymer poly(lacic-co-glycolic acid) PLGA has been extensively applied in tissue engineering as the scaffold materials, due to its good biocompatibility and biodegradability. However, the hydrophobility property of the biopolymer PLGA materials gives rise to the poor cell affinity on the surface and limits their application. Therefore, it is a hot topic to develop an efficient approach to increasing the hydrophilicity of PLGA materials.In this study, we introduced a class of hydrophobin to modify the biopolymer PLGA. Besides, we also investigated its application both in cell adhesion and the preparation of neural stem cells (NSCs) patterns which made the basis for the fabrication of the complex scaffold composed of NSCs and PLGA film.Hydrophobins are a group of amphiphilic and surface-active proteins found in filamentous fungi, which has been used to change the surface property of hydrophobic materials. The classâ…¡hydrophobin HFBI was used to modify electrospun PLGA scaffold and PGLA film. The static water contact angle of the HFBI-modified electrospun PLGA scaffold and PLGA film showed that their surface property conversed from hydrophobictiy to hydrophilicity. Collagen was modified on the HFBI-pretreated electrospun scaffold and the SEM image of the HFBI/collagen co-coated PLGA scaffold demonstrated that collagen could immobilized on the PLGA scaffold with high efficiency. When the HFBI/collagen co-coated PLGA scaffold was used as the substrate of culturing 293 T cells, the cells adhered and grew well on the surface. This result overcame the disadvantage of PLGA materials mentioned above and established the possibility of fabricating neural stem cells patterns on the PLGA film as well. Therefore, the micro-contact printing technology was used to prepare the serum patterns on the HFBI-modified PLGA film and the well-defined neural stem cells patterns was obtained by directly culturing cells on the PLGA film.HFBI modification of electrospun PLGA scaffold and PLGA film could not only efficiently change the hydrophobicity surface property and facilitate both the immoblization of biomolecular and cell adhesion on the surface, but also enlarge their application in the tissue engineering.