Relation Between The CD36 Genetic Polymorphism And Diabetic Nephropathy

Objective:To investigate the distribution of CD36 intron 3 [TG] repeat polymorphism in Type 2 diabetes mellitus (T2DM) patients of yanbian population and to expore the contribution of the genetic polymorphism with diabetic nephropathy (DN), study the genetic risk factors of DN. Methods:Genotypes of CD36 were typed in 193 T2DM patients and 80 normal controls by polymerase chain reaction(PCR) and PCR-direct sequencing; conducting a retrospective analysis of clinical features and biochemical indicators of 193 T2DM patients(91 patients with DN) With spss 17.0 packages. The distribution about groups of CD36 intron 3 [TG] repeat polymorphism was compared by x2 inspection,all biochemical indicators data of 193 T2DM patients were expressed with mean±standard deviation and were analysis using t test, frequence using x2 inspection; Logistic regression analysis revealed statistically significant difference (p<0.05). Results:(1) The distribution of CD36 intron 3 [TG] repeat polymorphism has no significant difference between normal control group and T2DM group (p>0.05). (2) The distributions of CD36 intron 3 [TG] repeat polymorphism exist statistically significant difference in T2DM between abnormal high-density lipoprotein cholesterol group(HDL-C<0.9 mmol/L) vs. normal group and abnormal low density lipoprotein-cholesterol (LDL-C＞3.12 mmol/L) vs. normal group (p<0.05). (3) The distribution of CD36 intron 3 [TG] repeat polymorphism exist statistically significant difference in T2DM between higher C-reactive protein (CRP>5.0mg/L) group and normal CRP group (p<0.05). (4) The distribution of CD36 intron 3 [TG] repeat polymorphism exist statistically significant difference in T2DM between higher body mass index(BMI>24.0kg/m2) group and normal BMI group (p<0.05). (5) Biochemistry data has revealed statistically significant difference in T2DM about process, hypertension, LDL, Cr (p< 0.05 or p<0.01), Age, Glucose, HbA1c CHO, TG, HDL, BMI has no statistically significant difference between DN group and non-DN group (all p>0.05) (6) Logistic regression analysis revealed that DN has close relation with long process,hypertension and the level of CRP(p<0.01), but no relation with Age, Glucose, HbA1c, Cr, CHO, TG, HDL, LDL and BMI(all p>0.05). Conclusion:(1) The distribution of CD36 intron 3 [TG] repeat polymorphism has relation with level of lower HDL-C, higher LDL-C,higher CRP and higher body mass index (BMI) in T2DM.(2)Long process, Hypertension, CRP and low density lipoprotein-cholesterol ascension are important risk factors for DN.