A Study On ECV304 Proliferation Induced By Candida Albicans

Objective: To study the effects of Candida albicans on the cell cycle distribution and proliferation of ECV304 cells in vitro.Methods:The line of human umbilical vein endothelial cell (ECV304) cultured in vitro were divided into four groups which were treated by Candida albicans supernatant, Candida albicans inactivated bacilli, supernatant and inactivated bacilli mixture, normal culture medium. We observed proliferous effect of ECV304 induced by supernatant, inactivated bacilli, supernatant and inactivated bacilli mixture using the methods of MTT, cell count, microscope and flow cytometry.Results:In the condition of different times and different culture concentrations, ECV304 cells incubated with 4-fold diluted Candida albicans supernatant for 48 hours increase the proliferation rate. The G0/G1 population of ECV304 cells decreased while S and G2/M cell population increased after incubated with Candida albicans supernatant for 40 hours,which showed significant increasing cell proliferation index (PI) (P<0.05)。The PI of the cells which treated by inactivated bacilli showed no significant change(P>0.05).Conclusion:Candida albicans could induce ECV304 cell proliferation which depends on the release of metabolic products of Candida albicans.