Study On The Fingerprints Of Plasma Amino Acids Of Patients With Heart Failure Of Xin-yang Deficiency Based On The Method Of RP-HPLC

BackgroundHeart Failure, HF, also named congestive heart failure, is a clinical and complicated syndrome that caused by myocarditis, coronary artery disease, hypertension and so on. This syndrome formed after the cardiac dysfunction and decreased cardiac output. Almost the cardiovascular diseases finally result in HF, which is the main cause of death. HF made a seriously influence on the life quality and longevity of the patient. High medical expense created a heavy burden to the family and the society. Therefore, the prevention and treatment of HF is always the focus of modern medical research. In recent years, along with the modernization of the Traditional Chinese Medicine (TCM) has became the focus of the problem, scholars have been trying to use the modern methodology to study TCM. The researches of the standardizations of syndromes differentiation is one of the hotspots.ObjectiveThe objective is to investigate using the Reversed-phase High performance Liquid Chromatography (RP-HPLC) to observe the feature of the fingerprints of plasma amino acids like Valine, Phenylalanine, Isoleucine, Leucine, Cysteine of patients with HF of Xin-yang Deficiency, At the same time, we want to find out the relationship between B-type natriuretic peptide (BNP), Partial Pressure of Carbon Dioxide (PaCO2), Partial Pressure of Oxygen (PaO2), Ejection Fractions (EF), E/A and HF of Xin-yang Deficiency。MethodsThis study is a cross-sectional study. It studies 28 inpatients with HF of Xin-yang Deficiency compared from out-patient clinic, Emergency Department, Observation Area, General Ward, Cardiovascular Department of Guangdong Provincial Hospital of TCM from December 2009 to April 2010. We took the blood sample and use the RP-HPLC to get the fingerprints of plasma amino acids to find out the characteristic of them. The data of the fingerprints, Xin-yang Deficiency syndrome scores, B-type natriuretic peptide (BNP), were observed and recorded to look for the correlation between amino acids and others.ResultsOne of the 28 patients with HF of Xin-yang Deficiency, who was a coma patient that was not able to be evaluated by Xin-yang Deficiency syndrome scores, was removed. The baseline of the remaining 27 patients (9 males,18 females) had no significant differences.The PaCO2, PaO2, EF and E/A had no significant correlation with Xin-yang Deficiency syndrome scores of those patients (r≈0). We get the fingerprints of Val, Phe, Ile, Leu by using the RP—HPLC to detect the 27 the plasma of the patients with HF of Xin-yang Deficiency, but not the Cys, since its content is too low to be detected. The whole process is about 20 minutes and the endogenous substances did not disturb the test. We drew the single scatter plot with the peak area of amino acid as independent variable, and the Xin-yang Deficiency syndrome scores as strain separately. We found out that the Xin-yang Deficiency syndrome score increase by Val and Leu increase and Phe and Ile decrease. But there is only one statistical significance between Phe and Xin-yang Deficiency syndrome score (P<0.05, Y =12.112+0.0000254XPh), no the other three, although all show the tend.ConclusionThere are advantages like high selectivity, rapid analysis, high sensitivity in testing the fingerprints of plasma amino acid base on the RP-HPLC. It is hard to detect the Cys because of its low content. But what is the reason need to be do some research. Phe showed the linear relationship with the Xin-yang Deficiency syndrome score. The other three amino acids showed the tend with the Xin-yang Deficiency syndrome score. Base on these, we suppose the amino acids fingerprints can be used in finding out the syndrome feature of the patients of HF with Xin-yang Deficiency.