The Observation Of Spermatogenic Function And Hormone Regulation On The Guilingji Taken By The Patients With Kidney Asthenia Oligoasthenospermia

ObjectivesThis subject focuses on the research of the Guilingji, which has spermatogenic function and hormone regulation on kidney deficiency oligoasthenospermia. And research it was from both clinical research and basic research. Intended to provide a kind of targeted, effective and safety treatment of therapy for oligoasthenospermia.MethodsThis research divides into the clinical observation and the animal expmentation. In the clinical observation part,36 suitable patients were allocated into groups according to medical sequencing, give Guilingji by oral. Each month is a treatment course, altogether three months. We observed curative effect in clinical and observed indexes before and after treatment. In the animal experiments part, We fed rats strictly according to the animal experiment requirement, and we control the rats's weight in 230±20g, the rats were divided into normal control group, model group, treatment group, each of group has 10 rats. According to the experimental method, we collected blood 5ml from the orbital venous plexus after 21 days, centrifugal;and after picking the blood, we executes the rats, selected the two-sided testicle, determined the observation targets. After that, all the data would be input into computer to establish database, used the SPSS13.0 statistics and perform statistical analysis of variance or T-test or Chi-square test. Result1. In the clinical observation, excluding 3 patients which didn't meet the test criteria of patients, the remaining 33 patients after treatment the total effective rate was 84.85%, a significant improvement compared with before.2. The clinical symptom of the oligoasthenospermia patients who is kidney yang deficiency in clinic is improved markly.3. In the clinical observation, the 33 patients after treatment, the sperm density, the sperm activity a level, a+b level, endocrine hormones T, LH, FSH significantly improved compared with before, the difference was statistically significant (P＜0.01)4. The linear regression analysis result is as follows:The sperm density increase with T a positive correlation, and LH, FSH is negatively correlated. The sperm activity a level and a+b level's enhancement and T become are being related, with LH is the inverse correlation, but FSH is actually not obvious relevance.5. In the animal experimentation, examines three groups of observation indexes with analysis of variance. Compared to the normal control group, the difference of the sperm activity, sperm density and endocrine hormone T, FSH and LH of the model group was statistically significant (P< 0.01), it shows that the model group was successful. Compared to the normal control group, the difference of the sperm activity, sperm density and endocrine hormone T, FSH and LH of the treatment group was statistically significant (P< 0.05), but the endocrine hormone T, FSH and LH was no statistically significant after treatment (P> 0.05), it shows that, the blood serum hormone level of SD rats close normal level after treatment.ConclusionThis study shows that Guilingji can improve not only the density and activity of the sperm, the endocrine hormone levels, but also the clinical symptom of the oligoasthenospermia patients who is kidney yang deficiency in clinic. It also been proved feasible in the SD rats which fed with Cyclophosphamide. But it whether used in patients with oral chemotherapy drugs to improve physical and spermatogenesis needs further study.