The Chinese Medicine Clinical Analysis Of Syndrome About The Acute Lung Injury And The Protective Function Of The Nervilia Fordii On Acute Lung Injury Of Rats

Objective:Investigates the clinical syndrome features of acute lung injury, summing up its internal rules to guide the clinical syndrome differentiation. On this basis, research into the effective protection of Nervilia fordii acute lung injury. And through observation of NF endotoxin induced acute lung injury in rat lung Aquaporin-land 5 expression, and discuss the mechanism of its protective effect.Method:190 cases observed symptoms, tongue and pulse of hospitalized patients with acute lung injury. The establishment of information database of clinical syndromes, the use of statistical methods, acute lung injury from a clinical point of view of changes of syndromes. There are 24 SD rats were randomly divided into 3 groups, Normal group (A groud), Nervilia fordii pre-intervention group (B groud), endotoxin model group (C groud). The use of the tongue at the end of intravenous endotoxin (5mg/kg), replication of acute lung injury model, Nervilia fordii administration deal with B groud, after 8h model test in lung wet/dry weight ratio (W/D) to observe the changes in lung water permeability and HE staining of lung tissue to observe pathological changes, immunohisto chemistry and RT-PCR test of lung tissue AQP-1, AQP-5 expression.Statistical methods:Use of descriptive statistics method to ali of the crowd and the analysis of the waiting card for; aoy acute damage to the sky and the rats in the protection of the single factor for the measurement of data by analysis.Results:Population characteristics:ALI occurs in middle-aged men,60 to 80 years is a good hair during ALI. Severe pulmonary infection and trauma are common risk factors. Distribution of clinical symptoms:shortness of breath, mental fatigue, weakness, cough, fever, lips cyanosis, lack of appetite for the main clinical symptoms of ALI medicine, which asthma, cough, fever, characterized by cyanosis lips symptoms; tongue mainly as follows:dark, light dark; tongue mainly as follows:yellow moss, sticky coating; pulse mainly:the number of slip, string, fine pulse. Basically reflected the main clinical symptoms of ALI, tongue, pulse situation. Syndrome Distribution:ALI distribution of the disease in patients with deficiency mixed evidence, evidence-based, only a minority of patients with disease of the direct performance of the simple deficiency. Evidence for toxic heat, phlegm, blood stasis syndrome of three main and most common deficiency of qi deficiency, yin deficiency, lung deficiency, the main deficiency.ALI model:after model of endotoxin, significant lung tissue swelling, lung congestion, we can see dark red spot-like lesions, lung W/D ratio was significantly increased in lung tissue observed under light microscope appear obvious pathological changes of lung injury, to prove the success of endotoxin induced ALI. Nervilia fordii protective effect on ALI:ALI After the modeling, and LPS model group, the protection of NF pre-lung tissue damage index decreased significantly reduce the performance of the lung tissue swelling, lung wet/dry weight ratio decreased permeability decreased;observed under the microscope, the structure of lung tissue damage, alveolar wall thickening, alveolar interval widened, cavity hemorrhage and exudation were reduced significantly, indicating lower degree of pathological damage, lung histopathology form significantly improved. Effective protection of NF mechanism of ALI:acute lung injury after endotoxin, endotoxin model of the lung AQP-1, AQP-5 protein expression was significantly reduced, with the LPS model group, pre-protection group of NF of AQP-1, AQP-5 protein expression was increased, thus confirming the sky Kwai on AQP-1, AQP-5 in regulating effect, note of NF can be adjusted by AQP-1, AQP-5 protein levels of, enhancement of pulmonary interstitial and alveolar fluid clearance in excess capacity, regulating water balance, improve lung water metabolism, thus effectively protect ALI.Conclusion:ALI TCM Syndromes:false and evil are actually the two ALI syndrome, is virtual in lung and spleen deficiency, both Qi-based, real evil to toxic heat, phlegm, blood stasis between the main cross junction. Early lesions to the evil reality-based, progression of the disease after the false is mixed as the main performance, and critical performance back to being off evil. Effective protection of NF acute lung injury, its protection mechanisms and through the promotion of NF lung AQP-1 and AQP-5 protein levels of up to increase alveolar fluid clearance rate and lung liquid transport, regulation of water homeostasis.