Study On Human Beta-Defensin-3,Histatin5 Against Candida Albicans Adherence To Human Oral Gingival Epithelium Cells

Purpose: This study is aimed to investigate the antifungal activity and mechanisms of the humanβ-defensin-3(HBD3) compared to human Histatin5 (Hst5) against Candida albicans, and their roles in inhibition of Candida albicans adherence to cultured human gingival keratinocytes in vitro.Method: Human gingival epithelium was cultured in vitro. The antifungal activity of the HBD3 and Hst5 against Candida albicans was tested by killing assays, and their inhibition of Candida albicans adherence to human oral epithelial cells was observed by transmission electron microscope further.Result: Synthetic HBD3 and Hst5 could kill most of the Candida albicans with at a related lower MFC50 (3.148±0.325ug/ml ; 9.85±1.158ug/ml, respectively), MFC90(14.42±6.604ug/ml;45.29±1.301ug/ml ,respectively). HBD3 showed stronger fungicidal activity against Candida albicans than Hst5 at the same concentration range(p=0.015).Observed by transmission electron microscope Candida albicans after incubation with HBD3 and Hst5 showed breakage of cell walls and membranes ,with leakage of cytoplasm material. In addition, both HBD3 and Hst5 could inhibit the yeasts attaching to human gingival epithelium.Conclusion: In the model of gingival epithelium in vitro, HBD3 and Hst5 both showed strong fungicidal activities against Candida albicans, rupture of cell walls and membranes, and efflux of cytoplasm material was observed, which might lead to cell death eventually. It suggested that both HBD3 and Hst5 could inhibit the yeasts attaching to human gingival epithelium.