| This paper aimed at the 20 batches of Chelidonium majus L. which were collected from different location in China. A new method of TLC was established to identify the six alkaloids (protopine, chelidonine, coptisine, stylopine, sanguinarine, berberine and chelerythrine). An UPLC method was developed to determine protopine, chelidonine, coptisine, stylopine, sanguinarine, berberine and chelerythrine and the total alkaloids were also' determined by vis-spectrophotometry. Besides the UPLC specific chromatogram of Chelidonium majus L. was established and analyze the alkaloids compositions by Q-TOF-MS. There were 17 common peaks. Fourteen alkaloids compositions were identified. Among them, 6 compositions were identified by direct comparison with the reference substances and others were identified by MS and MS2 data. These methods were useful for the evaluation of the quality of Chelidonium majus L. systematically and roundly.This dissertation is divided into two major parts.The first part was a detailed review about the latest research of quality evaluation for Chelidonium majus L., including herbal textual research, chemical constituents, pharmacological effects and quality standard.In the second part, there were seven chapters. Studied on the quality evaluation of Chelidonium majus L.In the first chapter, it was the informations of the investigated samples.In the second chapter, it was studied on the microscopic identification of Chelidonium majus L. and builded the [Identification](1) item.In the third chapter, it was studied on TLC. In order to obtain TLC chromatograms with better resolution for six alkaloids, different extracting solvents, chromatographic conditions and colorimetric methods were compared. Then, the TLC method used for Chelidonium majus L. identification has been established named [Identification](2).In the fourth chapter, it was studied on the content determination of water, ash content and extractives. Different methods were optimized. Then the method 1 (Drying in oven method) was used for determination of water in crude drugs containing no or scarcely any volatile constituents. The limitation of water was not exceed 13.0%, 12.0% for total ash, 0.6% for acid-insoluble ash and the limitation of extractives was not less than 17.0%.In the fifth chapter, it was studied on the content determination of alkaloids.This chapter included two parts.Part one was studied on the content determination of total alkaloids. It was performed on vis-spectrophotometry and the method was simple and showed good precision, repeatability and accuracy. The total alkaloids contents was23.25 mg/g～32.67mg/g and the average were 26.02mg/g.Part two was studied on the content determination of seven main active alkaloids. The UPLC method was performed on an ACQUITY UPLC BEH C18 (2.1×100 mm, 1.7μm) column. The mobile phase was composed of (A) acetonitrile and (B) 10 mmol/L mmonium acetate (adjusted to pH 3.0 with acetic acid) with a linear gradient elution. The flow rate of the mobile phase was 0.4 ml/min and the column temperature was maintained at 35℃. The injection volume was 1μl. Detection wavelength was set at 240 nm for protopine, chelidonine, stylopine, chelerythrine and 270nm for coptisine, sanguinarine, berberine. Good linear behaviors(r > 0.9992) over the investigated concentration ranges were observed for all the analytes. The recoveries (low, medium and high levels) were 95.55%～103.08%. The UPLC-PDA method was developed for simultaneously determination of 7 alkaloids in Chelidonium majus L., it showed good precision, repeatability, accuracy, fast analysis, acceptable resolution and less solvent consumption. The method was useful for authenticity controlling the quality of Chelidonium majus L.In the sixth chapter, the method of fingerprint of alkaloids in Chelidonium majus L. was setting up by UPLC-PDA. The analysis time was 30 min. The results of similarity evaluation showed that the similarity was more than 0.90. There were 17 common peaks. Besides the analysis of alkaloids compositions was performed by Q-TOF-MS. Fourteen alkaloids compositions were identified. Among them, 6 compositions were identified by direct comparison with the reference substances and others were identified by MS and MS2 data. And the fragmentation patterns of different alkaloids were inferred.In the seventh chapter, it was the protocol of the quality standard of Chelidonium majus L. which we builded based on the paper.
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