Effects And Mechanisms Of Bushen-Huoxue Decoction (BHD) On Embryonic Development In Endometriosis Model Rats

With its incidence rate increasing,Infertility and ache as the main clinical symptoms,endometriosis is called modern gynecology disease,in the sterile patient approximately 25%～35%is due to endometriosis.It has seriously affected the child-bearing age's work of woman,the study and the family life harmony,has become the influence to give birth one of most main gynecology department diseases. Through setting up endometriosis model by rat self-endometria transplantation,this subject was to observe effect of graafian follicle and embryo,immunization and reproduction related index were also detected.To investigate the effects and the mechanisms of Bushen-huoxue decoction(BHD) on regulating reproduction and immune function,searched scientific basis of BHD treating the sterile,displays Chinese medicine treatment characteristic and benefits sterility patient.1.EMS rats models were established by endometrium autotransplantation: Female virgin rats in estrus stage were induced by estrogen injection prior to operation. During the laparotomy,the right uterus was taken and its endometrium was implanted into between superficial fascia and muscular layer.Turned off the abdomen,the clean level environment raised for 4 weeks.2.The above model rats were randomized divided into 5 groups:model, high-dose,middle-dose,low-dose and positive drug(Gestrinone).Other sham operated rats were as control group.8 weeks after establishing model,examined by Radioimmunodetection of TNF-α,E₂,P in serum of rats and observed by immunohistochemisty of expression of VEGF,MVD,PR and graafian follicle.The changes of pregnancy rate,embryo was also observed.To study the effects and the mechanisms of BHD on adjust reproduction immunologic function in Endometriosis model Rats.1.Rat endometriosis models are Successful established by autologous transplantation.The TNF-α,E₂ level of model rats were elevated obviously and the expression of VEGF,MVD in the eutopic endometrial were also increase,It's similar to clinical pathological changes in human.2.The results of in vivo animal experiments showed that each dose group of BHD could decrease TNF-α,E₂ level in blood serum and the expression of VEGF,MVD in the eutopic endometrial.It showed that it can adjust the VEGF,TNF-αand MVD level,suppress ectopic tissue's vascularization to suppress the dystopia infection's growth,accordingly suppresses the growth,development of dystopia endometrium..By improving endometrium,promotioning Graafian follicle growth, thus achieves raises the rate of pregnancy,adjusts the reproduction function effectively.The pregnancy function of EMS rats can enhance effectively with BHD:Its function has following several aspects:controlling immunity action;improving organism internal secretion level;improving the eutopic tissues' environment;pressing the ovulation(possibly through to improve hypothalamic-pituitarygland-ovary axis, and ovary partial immunity,microcycle,metabolism micro environment,thus induced ovulation) and so on.