Optimal Production Of Mycelia And Polysaccharide In Submerged Cultivation Of Phellinus Linteus, And The Study Of Its Anti-tumor Effect

Phellinus Linteus is a medical mushroom which belongs to Phellinus.Phellinus Linteus has been described in Chinese herbal medicine literature to be effective on a diversity of diseases,including improving blood circulation and enhancing detoxication.With modern medicine and the continuous development of biotechnology,Phellinus Linteus has been investigated extensively for its extraordinary capacity of suppressing cancer and enhancing body immunity,which makes Phellinus Linteus a prospective candidate for developing novel anti-cancer compounds from natural resources.Recent studies have revealed that the Phellinus Linteus could improve the efficacy of chemotherapy drugs,when it worked together with the current chemotherapy drugs.This phenomenon have attracted more attentions from the researchers.The wild resources of Phellinus Linteus are very scarce and difficult to pick.Its yield is very small.Besides,due to the problems of the environmental pollution and so on,the researches of the rare medicinal fungus and the large-scale use have to face a too grave problem of resources.We solve the problem by use of the liquid submerged fermentation technology,and get the optimal nutritional requirements: 5%of Glucose,1.5%of Beef Extract,0.75%of Soybean Meal,0.06%of Magnesium Sulfate,0.06%of dipotassium hydrogen phosphate.The optimal submerged culture conditions:fermentation time,9 days;fermentation temperature, 27.5℃,initial pH,6.0;agitation rate,175rpm;inoculum volume,10%.Under the optimal culture condition,the maximum extracellular polysaccharide(EPS) concentration reached 2.651±0.075g / L which is about 1.5 times higher than that at the basal medium.The dry weight of mycelium reached 30.891±0.885g / L,which is about 2.5 times higher than that at the basal medium.The anti-tumor ability of EPS from Phellinus Linteus was confirmed.The tumor cell inhibitory rates could reach 34.5%when 0.75mg/ml EPS is added to culture medium of SGC-7901 cells for 72h.EPS at various concentration(0-0.5 mg/ml) could sensitize Dox-induced growthinhibition of SGC-7901 cells.The tumor-suppressor efficiency could be improved significantly when dox at nontoxcity concentration.When the dox at concentration of 0.1ug/ml added for 72h,the tumor cell inhibitory rates could reach 43.89%,but if adding EPS at concentration of 0.25mg/ml,the inhibitory rate of tumor cells could reach 66.13%.The experimental results of animals showed that,ethanol extract fraction(EEF) of Phellinus Linteus at concentration of 250mg/kg could reduce the gastric cancer significantly when it worked alone.At various concentrations(125,250 and 500 mg/kg),It could reduce the tumor significantly when combined with 30mg/kg cyclophosphamide.It was worth while noticing that the EEF had significantly effects of attenuating and profit-increasing to the chemotherapy drugcyclophosphamide...