| Hematopoietic stem cell automatic magnetic separation instrument, which we design and development, uses microcomputer and modern control technology. Through a magnetic nanometer granule and the CD34+ monoclonal antibody union to construct the magnetic nanoparticles biological molecular complexes, it makes the magnetic cell sorting solution for hematopoietic stem cell separation. Carrying on the full automatic control to the separation process and the pipe clean, may separate the stem cell batch, safely, precisely and fast. in order to isolate stem cells for the blood or cord blood from the isolated hematopoietic stem cells. Using this separation system, may cause to make the cost of blood stem cell separation reduce multiplied. Can be used in clinical as many tumors and autoimmune diseases in patients brought treatable and curable, use of the election system after the election, in which it allogeneic T cells can be removed to reduce GVHD occurred. Such large magnetic cell sorting adopts micro computer control and modern technology, on the cell separation and washing entire process control, batch, safe, efficient and rapid method to isolate cells.This paper mainly includes two aspects as follows:(1) Hematopoietic Stem Cell Sorter automatically using magnetic micro-computersand modern control technology, cell separation and washing the whole process automatic. According to the function and application of equipment, design of the hardware system of the apparatus including magnetic separation system, the sample delivery systems, computer control system and shell. Hardware Design of the main separation column completed development, pipeline design, casing design and so on. Hematopoietic Stem Cell Sorter automatically magnetic hardware system design a comprehensive, practical, as a medical research equipment In addition to superior performance, durability and other hard targets accurately, but also have the elegant, user-friendly features.(2) Hematopoietic Stem Cell Sorter automatically magnetic computer programmingthrough the control of fully automated, computer-controlled system can be divided into: control solenoid valve, magnetic motor control block, control of peristaltic pump, each control system for parts and refinement. Control system software to run when the requested equipment for each step and describe the precise expression. Instrument operation is divided into two major steps, one for system testing and pipe run washing steps, the step command steps to 34, spend 2 minutes. Second, cellular automata for sorting steps, the step command steps to 89, spent 20 minutes. Machines running the entire time is 25 minutes. Hematopoietic Stem Cell Sorter automatic magnetic separation wash system from the beginning of pre-filter, and then began sorting the labeled cells.(3) Hematopoietic Stem Cell Sorter automatically using magnetic micro-computers and modern control technology. Experiment through the magnetic nanoparticles with CD34 monoclonal antibody with magnetic nanoparticles to build - complex biological molecules, made of magnetic cell sorting solution. Most of magnetic nanoparticles in the size 50-80nm, narrow distribution of particles is a hybrid package, which packages a number of magnetic nanoparticles, dispersion also better reunion phenomenon is not very serious, a good super-paramagnetic. Hematopoietic stem cells in the automatic magnetic separation apparatus for separation and enrichment of hematopoietic stem cells, have been a better test results, after the separation by flow cytometry to obtain more than 50%purity, more than 40% separation efficiency, good effect in vitro amplification with more practical. |
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