Lymphocyte Subsets Analysis Of Children With Obstructive Sleep Apnea-hypopnea Syndrome Before And After Operation

Obstructive sleep apnea-hypopnea syndrome (OSAHS)is the most important research areas of sleep medicine , the dangers of OSAHS in children has aroused widespread attention in recent years, the current study shows that it can affect multiple systems and organ function, and may cause a serious impact on growth and development of patients, while the immune system function whether is affected was clearly. The major reason cause children OSAHS is the adenoidal and tonsillar hypertrophy ,and yet is proved that adenotonsillectomy is the first-line treatment of children OSAHS , but there is no consistent conclusion were reached whether adenotonsillectomy can improve immune system function in OSAHS children .ObjectiveThrough flow cytometry to detect peripheral blood lymphocyte subsets levels and the ratio of obstructive sleep apnea hypopnea syndrome children before and after Adenotonsillectomy.To explore the immunity change of children with OSAHS before and 3 to 6 months After Adenotonsillectomy.Methods30 OSAHS children diagnosed by overnight sleep monitoring, venous blood simples were taken from the patients 24～48 h prior to and 3-6 months after the surgery. lymphocyte subsets were testing by Becton Dickinson﹝BD﹞FACS Calibur flow cytometry. The levels of CD3 + , CD4 +, CD8 +, CD19+, CD25 + and CD16 + + CD56 + were mesured. And at the same time to select 30 children excluded related diseases to compose the control group, the age and sex was matched. Peripheral venous blood were mixing with anticoagulant, lymphocyte subsets analyze use the Becton Dickinson FACS Calibur flow cytometer.ResultsIt was found that the levels of CD4+ was lower than the control group in the pre-operative period, and CD4+/ CD8+ was lower too, while the CD8+ was higher than the control group in the pre-operative period, the differences were significant (p <0.05).There were statistically significant differences between pre- and post-operative levels of CD4+ ,CD8+ .The CD4+ CD25+ was higher in the pre- and post-operative OSAHS child than the control group .ConclusionThe lymphocyte subsets of OSAHS children significantly changed, and therefore may lead to changes in immune function in children, while 3～6 month after adenotonsillectomy, the proportion of lymphocyte subsets returned to normal. This explains adenotonsillectomy can improve the immune function of the patients.