Studies On The Extraction, Purification And Weight Reduction On Pinolenic Acid In Pine Nut Oil

The purpose of this paper was to study on pine nuts oil and pinolenic acid, and to provide basal parameters for their exploration.The results of Soxhlet extraction method showed that the oil content of the pine nut was 65.5%. Ultrasound wave method is prior to thermal reflux method on pine seed oil extraction. And the highest extract rate was 61.35%. The acid value, saponification number and POV were detected in the paper. The composition of fatty acids in pine nut oil was detected by GC-MS. The oil consis maily of palmitic acid, stearic acid, oleic acid, linoleic acid, pinolenic acid, 9,12-ectadecadienoic acid, cis-8,ll,14-eicosatrienoic acid, arachidonic acid and two types of unknown acid. GC result showed pinolenic acid in pine nut oil was 10.60%.Pinolenic acid were separated and purified from the pine nuts via urea clathration. The orthogonal tests showed the best condition of pinolenic acid: fatty acids: urea, 1: 1.5(W/W); urea: ethanol, 1:8(W/V); the temperature of crystallization: 0℃; crystallization time: 24h. On this condition, the concentration of pinolenic acid increased to 67.08%.The effect of Pinolenic acid on the weight reduction of nutritive obesity mice was observed. Nutritive obesity model in mice was made by high nutritive diet, and treated with pinolenic acid at the dose of 2,4 and 8 g/kg·d^-1 and the positive control group was treated with 8 g/kg·d^-1 drog. The body weight, Lee's index, fat index, liver index, kidney index, lipids and Glu in serum . the volume and the maximum diameter of fat cells were determined. The results showed that pinolenic acid could decrease body weight, Lee's index, fat index, liver index, kidney index and Glu in serum of nutritive obesity mice obviously (p<0.05). The TC level in low dose group decreased significantly (p<0.01); in middle dose group the TG level decreased and HDL-C level increased significantly (p<0.01); in high dose group LDL-C level and Glu level decreased significantly (p<0.01).One word, the pine nut oil could regard as not only good edible oil but also newly health oil. And pinolenic acid extracted from pine nut oil also has high utilization.