Protective Effects Of Cannabidiol On Injury Of PC12 Cells Induced By Aβ_25-35

ObjectiveAlzheimer's Desease(AD) is the most common central neurodegenerative disease. It is characterized by memory losss,confusion,and a variety of cognitive disabilities. There was found neuofibrillary tangle and senile plaque from Pathology.β-amyloid has been proved to play a vital role in AD and be capable of inducing cell apoptosis presented by charaeteristic changes.It is an ideal model of the AD research in vitro to have Aβ_25-35 induce the PC12 cell damage and establish the AD cell model.Cannabis sativa,as a kind of traditional plants,has been reorded long time ago. The main chemical composition in the annabinoids are△g-tetrahydrocannabinol and cannabidiol.In contrast to△g-THC,cannabidiol is a non-psychoactive component from Cannabis sativa.It exerts a plethora of pharmacological effects,including anti-convulsive,sedative,hypnotic,anti-psychotic,anti-nausea and anti-inflammatory actions.Cannabidiol is a potent antioxidant compound and it has been recently proposed to have a neuroprotective role.In our experiment,we set up a cell model through using PC 12 cells incubated by Aβwhich is pretreated by cannabidiol.We observe the effect of cannabidiol on Aβ_25-35 induced survivalrate,morphological feature of cells,apoptotic rate and activity of SOD. The present study aimed to observe the protective effects of cannabidiol on the damage of PC12 cells induced byβ-amyloid peptide(Aβ_25-35).MethodsThe PC12 cells were pretreated with cannabidiol(0.1μmol/L,0.5μmol/L, 1μmol/L,5μmol/L),for 30 minutes,then incubated with Aβ_25-35(Aβ_25-35 was aggregated 37℃for 2 days).In every experiment there were normal control group (0μmol/L Aβ_25-35),treatment group(10μmol/L Aβ_25-35) and drug protective group (CBD+Aβ_25-35). 1.MTT method was used to detect cell viability.The primary culture mediumin 96-well culture plate was replaced by fresh culture medium containing MTT20μl,then moculated for 4hours,150μl DMSO was added to terminate the reaction.Automatic micro-titre board reading meter was used to detect the absorbency at 57Onm(OD value),and cell viability was expressed by the percentage of sample OD value and control OD value.2.The morphological change of PC 12 was observed by HEstaining.3.The apoptosis of PC12 cells was detected by flow cytometry.PC12 cells in 25 ml culture bottle were harvested.Apoptosis rate was detected by FCM with P1 staining.4.Evaluated the level of the activites of SOD.Results1.Cell survival rate:Cell survival rate was found declined in PC 12 cells induced byβ-amyloid_25-35.OD value was significantly higher in cannabidiol group than those in Aβgroup.cannabidiol significantly increased cell survival rate and it was concentretion -dependent.2.Morphological observation showed cell number was more and less injured cells. In cannabidiol group than those in Aβgroup.3.The number of apoptosis cell significantly increased in Aβtreatment group.The number of apoptosis cell in cannabidiol treatment group reduced greatly compared with that of Aβgroup.4.Activities of SOD were decreased by treatment Aβ_25-35.Cannabidiol treatment group could increase the activities of SOD and there was a significant difference compared with Aβtreatment group.ConclusionsCannabidiol protects PC12 cells against Aβ_25-35-induced of viability and increase of apoptotic rate,increase of activities of SOD.Cannabidiol could play the Protective role in PC12 cells injury induced by Aβ_25-35.