The Research Of Possibility Using IL-6 And TNF-α Content Of Saliva As Marker Of Molecular Diagnosis In Severe Periodontitis

Many studies have proved that there exist amount of inflammatory mediators in the gingival cervical fluid and gingival of the periodontitis patients. Present studies mainly focused on the gingival cervicular fluid (GCF),to evaluate the severity and stage of the disease by detecting cytokines and proteins in gingival crevicular fluid. There are some disadvantages: 1. GCF was easily polluted by saliva, and the method may not acquire an accurate result; 2. When and how to insert filter strips should be controlled precisely. Saliva samples are easily collected and contained lots of compositions which can be used as sensitive index to detect periodontal diseases and evaluate the healing results in periodontitis. GCF-derived mediators in saliva may be valuable indicators of the diagnosis of periodontitis.Objective:IL-6(interleukin-6 IL-6)and TNF-α(tumor necrosis factor-alpha)were detected in unstimulated whole saliva samples of patients with severe chronic periodontitis before and after treatment. Compared to the health adults, the association between periodontitis and Saliva IL-6 and TNF-αcontent was determined. And we analyzed the possibility of IL-6 and TNF-αcontent to be the molecular marker of periodontitis.Materials and methods:In this study, unstimulated whole saliva samples of 36 patients with severe chronic periodontitis and samples of 26 healthy human beings were collected. The male-female ratio was 1:1 in each sample(patients 18, normal control 13). ELISA was used to detect IL-6 and TNF-αcontents of unstimulated whole saliva samples in patients with severe chronic periodontitis in clinic. To exclude the effects of other physical indexes on the results,periphereal blood of each sample was tested routinely and the samples with increased WBC were abandoned. By this way we could exclude inflammation in other parts. The data (such as gender, age, WBC) of each sample selected was analyzed with paired-samples t test.Results:The results showed that IL-6 and TNF-αcontents in saliva of patients with severe periodontitis were significantly higher than normal samples and had no relation to gender and age.Conclusion:1. There are significant differences between patients with periodontitis and controlled group in contents of IL-6 and TNF-αin saliva. It can become a potential molecular diagnostic marker in the future.2. There are no gender differences in IL-6 and TNF-αcontents of saliva..3. Predictive value of leukocyte in blood routine test can not affect contents of IL-6 and TNF-αin saliva.