Expression Of Human Leukocyte Antigen-G In Ovarian Cancer

Objective: Ovarian cancer is one of the most prevalent gynecologic cancers in women presently and it is still lack of effective early diagnosis and treatment. Immune escape plays an important role in the incidence of cancer. A tumor cells to evade immune surveillance by upregulating the expression of human leukocyte antigen - G (HLA-G) is one of the mechanisms. HLA-G is a non-classical Major Histocompatibility Complex (MHC) I molecule, it induced the maternal-fetal interface of the immune escape, and in the reproductive, cancer , organ transplantation, infection and autoimmune diseases have played an important immunosuppressive effects.This study through immunohistochemistry and RT-PCR method to detect HLA-G in the protein and mRNA expression levels in ovarian cancer is to explore its role in the occurrence and the development process ovarian malignancy, and its clinical significance.Methods: The immunohistochemical SP method and reverse transcriptase-polymerase china reaction (RT-PCR) analysis of fresh ovarian benign and malignant tumor tissues of HLA-G in protein and gene expression levels. (1) Clinically diagnosed primary ovarian cancer of 45 cases, 36 cases of ovarian benign organizations as control. Application of anti-HLA-G monoclonal antibody 4H84, detection of benign and malignant ovarian tumor tissues paraffin section of HLA-G protein expression by immunohistochemistry. (2) Intraoperative immediately drawn fresh tissue of ovarian tumors 28 cases, of which 15 cases of malignant tumors, 13 cases of benign tumors, eight villus cases of voluntary abortion line of normal pregnancy 6 to 8 weeks pregnant women as positive control, placed in sterile tubes frozen in liquid nitrogen cryopreservation to be detected. Grinding tissues into a tissues homogenate, applications TRIZOL extracted RNA from tissues, the application of RT-PCR detection of benign and malignant ovarian tumor tissues of HLA-G mRNA expression.Results: (1) Cell membrane and cytoplasm granules appear brown or brown granules for staining positive cells, according to the degree of tumor cells staining and staining cells integrated score, Immunohistochemistry showed that ovarian cancer in 30 cases of expression of HLA-G positive expression rate of 66 percent, the corresponding 36 cases of ovarian benign tumor in 4 cases (11.11 percent) of positive expression of HLA-G.Comparison of ovarian cancer and benign ovarian tumor tissues expression of HLA-G-positive rate, the difference was statistically significant (P<0. 01) . (2) the HLA-G molecule positive rate differences were statistically significant (P<0.05) , between ovarian serous cystadenocarcinoma, ovarian mucinous cystadeno-carcinoma, ovarian endometrioid carcinoma, however, it is not significantly associated with age, tumor stage, grade and lymph node metastasis.(3) RT-PCR analysis demonstrated the presence of HLA-G mRNA in ovarian malignant tumor tissues, but HLA-G mRNA was not detected in benign ovarian tumor tissues and ovarian tumor-like lesions.Conclusions: HLA-G mRNA and HLA-G protein is ectopically expressed on ovarian malignant tumor tissues, which indicated that immunosuppression plays an important role in the occurrence and development of ovarian cancer cells.