Expression And Significance Of Cyclin D1,Cyclin E And P27 In Transitional Cell Carcinoma Of Renal Pelvis

Objective: The nature of malignant tumor is cell cycle out of control, which cause uncontrolled cell dividing and proliferation. Cell cycle regulatory factor D1 (cyclin D1) and Cell cycle regulatory factor E (cyclin E) are involved in the initiating of DNA duplication. P27 is an important cell cycle negative regulation protein, involved in blocking cell cycle in G1 phase. In this study, we investigate the expression and clinical significance of cyclin D1, cyclin E and p27 in transitional cell carcinoma of renal pelvis.Method: In total, 42 paraffin embedded specimen was studied in this group, with complete clinical data and each is confirmed by pathological test. Five serial section of each specimen were prepared. One section was performed by hematoxylin-eosin staining. Three were performed by immunohistochemical staining, with mouse-anti-human monoclonal p27, cyclin D1, cyclin E antibody as first antibody respectively. One was processed by PBS as negative control. Eight normal renal pelvis tissues were compared as negative control. The staining information was studied under microscope. Ten vision field were selected randomly. Positive result was determined by positive cell counting.Results: cyclin D1 and cyclin E were positive in the nucleus of the cell. In this study, we find cyclin D1 is weakly positive in normal pelvis tissue and ureter tissue, while strong positive in transitional cell carcinoma of renal pelvis, depending on the pathological stage and histological grade.p27 was also positive expressed in the nucleus of the cell. In this study, we find p27 expression has statistical significance between transitional cell carcinoma and normal tissue. p27 was positive in normal tissue, while less positive in transitional cell carcinoma, positively correlated with the pathological stage and histological grade. cyclin D1 expression expression is negatively related with p27 in differentiation and TNM stage of transitional cell carcinoma. cyclin E expression expression is negatively related with p27 in differentiation and TNM stage of transitional cell carcinoma.Conclusion: In this study, we find p27 expression is negatively related with cyclin D1 and cyclin E expression. In transitional cell carcinoma, p27 expression is less positive, while cyclin D1 and cyclin E expression is strong positive. It indicates that p27 deactivation and cyclin D1 and cyclin E overexpression are all involved in the tumorigenesis of transitional cell carcinoma. Combined detections of p27, cyclin D1 and cyclin E may help reveal the malignancy of transitional cell carcinoma and predict the prognosis.