Studies On Etraction & Purification Processes Of Total Alkloids Of Eomecon Chinantha Hance

Eomecon chinantha Hance is a many-year living herb and a species of unique category and unique genus in China,extensively distributing in some southern provinces such as Zhejiang,Jiangxi,Sichuang,Hunan etc.Its root and rhizoma are used in medicine.In the traditional Chinese medicine,it can clear away hot and toxic.The content of Chelerythrine is the most.The modern pharmacological study indicates that the alkaliods can block cardiac cell passages and prevent various experimental virulent cite cardiac arrhythmia.It also inhibits the action of enzymes and WBC in human body and animal cells fluences cell's function,and destroys bacterium and virus.Therefore,the aim of the study on the extraction and purification processes was to provide scientific basis for the manufacture of total Eomecon chinantha Hance alkaloids.On the bases of previous researches into Eomecon chinantha Hance and on consideration of specific resources of the laboratory,this article optimized the parameters of reflux extraction which was screened by the optimization design. The purification process of total alkaloids with apply medical material was generally studied by taking content of total Eomecon chinantha Hance alkaloids research concentrate extract in different degree get separation systematical,compare content of total Eomecon chinantha Hancecontention alkaloids use different organic solvent extraction and salt fractionation transaction extractum liquidum,consideration purify effect of Chelerythrine and Sanguinarine use recrystallisation.The results were as follow:1.The optimal parameters of the reflux extraction were that Eomecon chinantha Hance refluxed in the 95%EtOH(3-folds) for 1.5 h,3 times in all.2.The investigation result of total Eomecon chinantha Hance contention alkaloids by concentrate extract in different degree;the purify of total Eomecon chinantha Hancecontention alkaloids is most in degree 1:1.3.The result of compare content of total Eomecon chinantha Hance contention alkaloids use different organic solvent extraction and salt fractionation transaction extractum liquidum;salt fractionation is better than organic solvent transaction extractum liquidum.4.use 95%EtOH repeat recrystallization separate matter get Chelerythrine and Sanguinarine total count 92.77%...