| In the recent years, capillary electrophoresis (CE) with electro-chemiluminescence (ECL) based on tris(2,2'-bipyridyl) ruthenium(Ⅱ) [Ru(bpy)32+] has been used in the field of drug analysis, clinical analysis, physiological research, pharmacological research and toxicological research. In the study, capillary electrophoresis(CE) with Electrochemiluminescence(ECL) shows its special merit of rapid speed, high efficiency and great sensitivity. And this thesis is concentrated on the application of Ru(bpy)32+ Electrochemiluminescence(ECL) coupled with capillary electrophoresis(CE) technique in analysis of pharmaceuticals as well as clinical analysis, as described below:In chapter 1, we introduced the principles of CE and ECL firstly and described their classifications and concerned techniques. In addition, the general introduction was given in the application of CE-ECL in pharmaceutical analysis as as well clinical analysis in this chapter. And the prospects of CE-ECL technique were forecasted.In chapter 2, based on the ECL enhancement of Ru(bpy)32+ with the betahistine analyte, capillary electrophoresis(CE) coupled with electrochemiluminescence(ECL) using Ru(bpy)32+ reagent has been developed in the determination of betahistine hydrochloride in the commercial tablet. This CE-ECL detection method has high sensitivity, good selectivity and reproducibility for betahistine hydrochloride analysis. Under the optimized conditions, the linear concentration of betahistine hydrochloride ranged from 5×10-7-1.5×10-4 mol/L(r = 0.9996), and the limit of detection was 5×10-8mol/L(S/N=3).In chapter 3, Capillary zone electrophoresis(CZE) coupled with Ru(bpy)32+ based on end-column electrogenerated chemiluminescence(ECL) has been utilized to detect bisoprolol in drugs and tablets after its separation from metoprolol. Tetrahydrofuran was used as an additive in the running buffer to obtain the absolute ECL peak of bisoprolol. Bisoprolol reacts as a co-reactant in Ru(bpy)32+ electrochemiluminescence system. Under the optimized experimental conditions, bisoprolol was separated successfully and efficiently from metoprolol and other co-existed materials in tablets and urine samples. The ECL intensity of Ru(bpy)32+ -bisoprolol based system is linear with the concentration of bisoprolol from 1.5μmol/L to 0.3mmol/L with a detection limit of 0.3μmol/L. Relative standard derivations of the ECL intensity are 2.58% for the detection of 15μmol/L bisoprolol. This method is a simple, rapid, selective, and sensitive. It was applied successfully for the monitoring of bisoprolol in market available tablets and human urine samples.In chapter 4, we developed a new fast capillary electrophoresis(CE) with cysteine(Cys) electrochemiluminescence(ECL) method to determine cysteine(Cys), ascorbic acid(AA) and uric acid(UA) which are the important biocomponents in human biological system by using antioxidants' (uric acid and ascorbic acid) inhibition based on Ru(bpy)32+ - tripropylamine(TPrA) system for the simultaneous determination of these three components concentration in human body fluid (serum and urine). Under the optimized conditions, we could achieve the linear range from 2.0×10-6 to 2.0×10-4 mol/L for three of them and detection limit of 0.4μmol/L for ascorbic acid(AA), 0.02μmol/L for uric acid(UA) and 0.5μmol/L for cysteine(Cys) (S/N=3). Relative standard derivations of the ECL intensity were 3.21 %, 2.46 % and 2.94%for ascorbic acid(AA), uric acid(UA) and cysteine(Cys), respectively. |
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