Association Of Genetic Polymorphisms In CD147 Gene With Psoriasis

Objective: To investigate the association of CD147 genetic polymorphisms with psoriasis vulgaris susceptibility in Chinese Han by a case-control study.Methods: Peripheral blood mononuclear cell (PBMC) were isolated from eight patients with psoriasis vulgaris and eight normal person by density gradient centrifugation. The subtype of CD147 mRNA were watched by semi-quantitive reverse transcriptase-poly merase chain reaction (RT-PCR). The change expression of CD147 mRNA were mesured by realtime PCR. By using the public data deposited in the NCBI database, single nucleotide polymorphisms (SNPs) at CD147 locus in Beijing Chinese Han were recorded. Rs8259 was selected for further study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to genotype the candidate SNPs. A case-control study of 164 newly diagnosed psoriasis patients and 175 normal controls of Han nationality recruited from Hunan province was carried out.Results:1. CD147 mRNA is tranformated as one of the subtypes BSG-2 in PBMC.2. The CD147 mRNA expression level of psoriasis patients in the PBMCs is higher than that of normal subjects. 3. Ten common SNPs were recorded in the NCBI database for the Chinese population.4. Genotype frequencies of BSG-2 T10292A AA, AT and TT genotypes were 28.05%, 62.80%, 9.14% respectively in psoriasis patients. The frequencies of three different genotypes are26.86%, 51.43%, 21.71% respectively in control subjects. Significant distinction was observed in genotype distribution of T10292A polymorphism (P=0.005) between cases and controls.5. The allele T of CD147 T10292A is match with miR-492.Conclusion: 1. CD147 mRNA is tranformated as one of the subtypes BSG-2 in PBMC.2.BSG-2 genetic polymorphisms are associated with psoriasis susceptibility in Chinese, and BSG-2 is an important susceptible gene in psoriasis.