Expression And Clinical Significance Of HSP70 And Apaf-1 In Pathological Scars

ObjectiveTo detect expression of HSP70 and Apaf-1 in pathological scar and normal skin tissue,and explore their influence in formation of pathological scar.Methods62 specimens of scars(including 18 hypertrophic scars,11 keloids, 16 surgical scars and 17 normal skin tissues) were collected from the Department of Burn and Plastic Surgery,Xiangya second Hospital of Central South University from March to December,2008.They were assayed by using immunohistochemical method(SP method) for detection of HSP70 and Apaf-1 protein expression level.The average surface density of HSPT0,Apaf-1 were analysed to study the difference and dependablity in pathological scar and normal skin tissue.Results1.The average surface density of HSPT0 in hypertrophic scar and keloid were higher than normal skin and flat scar.P＜0.05.There was statistical significance.2.The average surface density of Apaf-1 in hypertrophic scar and keloid were lower than normal skin.P＜0.05.There was statistical significance.3.There were a inverse correlation between HSP70 and Apaf-1 in hypertrophic scar,keloid,surgical scar and normal skin tissue.R=-0.151.Conclusions1.The overexpression of HSP70 in pathological scar,keloid suggested that HSP70 may facilitate the formation of pathological scar.2.The low-expression of Apaf-1 in pathological scar,keloid may be associated with insufficient apoptosis of scar tissue.3.Intervening Gene Expression of HSP70 and Apaf-1 in scar tissue may provide one method for prevention and treatment of pathological scar.