The Effects Of RNA Interference By Silencing Vascular Endothelial Growth Factor C Gene On T24 Cells Of Human Bladder Cancer

Objective: To construct VEGF-C gene of the siRNA lentiviral vector and observe the effects of RNA interference on T24 cells of human bladder cancer.Methods: The siRNA against VEGF-C gene was designed and transfected into T24 cells, the expression of VEGF-C gene was measured by using RT-PCR and ELISA. Cell proliferation was measured with MTT assay.Results: The positive clones were sequenced and the results showed that they were correct genes, and the genes can be detected in the expression level of cells. The results of Western Blot showed that PSC1261 could significantly knock down the target gene. And PSC1261 was the best target. Fluorescence observations showed that the efficiency of T24 cells infected was more than 80%. RT-PCR manifested that the targets significantly knocked down VEGF-C gene on T24 cells (p <0.05): only 15% VEGF-C mRNA expression was observed in KD group relative to NC group. ELISA indicated RNAi could significantly reduce the expression of T24 cells (P <0.05). MTT showed that RNAi inhibited the proliferation of T24 cell (P <0.05).Conclusion: A VEGF-C gene targeting eukaryotic expression vector RNAi was successfully constructed and it could effectively inhibite VEGF-C on T24 cells. It is the basis for further study the VEGF-C gene in the course of bladder cancer lymphangiogenesis, and lymph node metastasis.