Apoptosis And Growth Arrest Induced By Cisplatin And Adriamycin In U2-OS Cells Associated With The Genes Expression In Vitro

Objective: To investigate the expression of p53﹑bcl-2 and c-myc and its effects on cell cycle arrest and apoptosis induced by cisplatin and adriamycin, the chemotherapeutics inU2-OS cells.Methods:After the treatment of 0.3μg/ml, 3.0μg/ml, 30.0μg/ml cisplatin and 0.06μg/ml, 0.6μg/ml, 6.0μg/ml adriamycin for 2h, we keep on cultivate U2-OS cells with fresh medium in 0, 6, 12, 24, 48h. Then MTT were used to evaluate cell proliferation in vitro. The expression of wild-type p53, bcl-2,c-myc were detected by RT-PCR and Western blot, Flow Cytometry (FCM) was employed for examining the cell cycle after cultivate for 6h, 24h.Results: After damage by different dencity of cisplatin and adriamycin, U2-OS cell were cultivated for 6h, FCM show that cell in G1 phase were incresed obviously, the ratio were 67.97%, 79.64%, 78.09%, 80.21%, 78.25℅, 79.13℅ and 79.92℅.The expression of p53 were up-regulate, bcl-2 and c-myc expression were unchangeable, the inhibite of cell grow were not manifest in statistics. After cultivate for 24h, the inhibitory rate of cell growth of group②③④⑤⑥⑦were 31.58%, 44.12%, 59.46%, 23.40%, 40.31% and 57.56%. Group②③④were different in statistics, Group⑤⑥⑦were different in statistics by contrast with each other. The ratio of apoptosis by FCM were 10.04%, 14.55%, cell cycle arrest in G2 phase. The expression of p53, bcl-2, c-myc was different obviously in statistics of group②③④, the expression of p53, bcl-2, c-myc was different obviously in statistics of group⑤⑥⑦. After cultivate for 48h, the inhibitory rate of cell growth was increased..Conclusions:The U2-OS cells were damaged by different dencity of cisplatin and adriamycin in short time(2h), the inhibition of cell grow was also manifest. The effection were related with the diversify of the expression of p53, bcl-2, c-myc after DNA damage. The diversify of p53 induced G1 cell cycle arrest after 6h, and after 24h, the diversify of p53, bcl-2, c-myc induced G2 cell cycle arrest and apoptosis, the change was related with drug concentration.