The Study On The Expression Of Pro-oncogene Pim-3 And Its Protein In Human Rectal Adenocarcinomas

Objective: To detect the expression of pro-oncogene Pim-3 and its protein in human rectal adenocarcinomas,evaluate its role in the carcinogenesis and progression of tumor and clinical significance.Methods:Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect the expression of Pim-3 and its proten in 30 rectal adenocarcinomas and the corresponding adjacent normal tissues and 30 normal rectum mucosas stored by liguid nitrogen ,and analyze their relations with clinicopahtological characteristics.Results: Pim-3 gene was aberrant expressed in both human rectal cancer tissue and the corresponding adjacent normal tissue,and the expression in rectal carcima tissue was more than in the corresponding adjacent normal tissue(P<0.05).The normal rectum mucosa had no expression of Pim-3 gene(P<0.01).Of the 30 rectal adenocarcinomas,pim-3 protein was detected in 19 cases;of the 30 corresponding adjacent normal tissue,8 cases had pim-3 protein expression(P<0.01).The aberrent expression of Pim-3 and its protein in rectal adenocarcinomas has no relationship with some clinicopathological characteristics,such as:gender,age,tumor size,the depth of tumor invasion(P>0.05),but were significantly related to the tumor differention,lymph node metastasis and liver metastasis(P<0.05).Conclusion: Pro-oncogene Pim-3 and its protein are aberrant expressed in human rectal cancer and maybe play an important role in the carciongenesis and progression of tumor.Pim-3 gene may be used as a putative prognostic indicator of rectal cancer.