Effect Of Jiejufushenning On Expression Of PKC,PDGF-β,VEGF And BMP-7 In Diabetic Nephropathy Rats

Objective:To investigate the effect of Jiejufushenning(JJFSN) on expression of renal protein kinase C(PKC),renal vascular endothelial growth factor(VEGF),renal platelet-derived growth factorβ(PDGF-β) and renal bone morphogenetic protein-7(BMP-7) in diabetic nephropathy rats and evaluate the protective effects of Jiejufushenning(JJFSN) on renal lesion in diabetic rats.Methods:The diabetic SD rats induced by STZ were divided into four groups: diabetic control group, Jiejufushenning group,Irbesartan group and Jiejufushenning combined with Irbesartan group,and the normal control group. After experimental rats in every group were respectively treated for 12 weeks by above intervention methods.The relative kidney weight, blood sugar,urea nitrogen, serum creatinine, and urinary albumin excretion rate (UAER) were detected by routine analysis methods, and PKC,VEGF and BMP-7 in renal tissue was detected by immunohistochemical techniques,and PDGF-βin renal tissue was detected by Western blot,and pathologic variation of kidney was observed by microscope.Results:The expression of renal PKC,VEGF and PDGF-βwas increased in diabetic rats . The expression of renal BMP-7 was decreased in diabetic rats.The pathologic variation of kidney was noticeably changed, and the blood sugar,UAER,urea nitrogen,serum creatinine and relative kidney weight were significantly increased in diabetic rats. While in addition to the expression of renal BMP-7 ,the above indexes in JJFSN group,Irbesartan group and JJFSN combined with Irbesartan group were significantly lower than in diabetic control group (P<0.05), the expression of renal cortex BMP-7 in JJFSN group, Irbesartan group and JJFSN combined with Irbesartan group were significantly higher than in diabetic control group(P<0.05), and pathologic variation of kidney was improved,and the improvement of all indexes in JJFSN combined with Irbesartan group was most significant (P<0.05). Conclusion:JJFSN has the protective effects on renal lesion in diabetic rats. The mechanism of the protective effects of JJFSN on renal lesion may be downregulating the expression of PKC,VEGF and PDGF-β,and increasing the level of BMP-7 in the renal tissue.