Expression Of Adiponectin And Its Receptors In Human Serum, Follicular Fluid And Granulosa Cells And The Correlations With The Outcome Of IVF-ET

PartⅠExpression of adiponectin and its receptors in obese women during IVF cyclesobjective: To compare adiponectin levels in serum and follicular fluid (FF) and the expression of mRNA of adiponectin and its receptors (AdipoR1/AdipoR2) in follicular luteinized granulosa cells (GCs) from women with obesity and controls during IVF-ET cycles. And also, to investigate the relationship between the adiponectin or its receptors levels and obesity, sex hormone, follicular maturation and the outcome of IVF-ET.Methods: Serum, FF and GCs were obtained from a total of 71 women, with 31 obese women (BMI>24kg/m2) and 40 normal weight women (BMI≥18.5kg/m2 and BMI≤24kg/m2), during the oocyte retrieval. The adiponectin concentration was determined by ELISA, Sex hormone levels were by chemiluminescent immunoassay. The mRNA expression of adiponectin and its recepters were measured by RT-PCR.Results: serum adiponectin levels and mRNA expression of its receptors were significantly lower in obese women than in women with normal weight (P<0.001 and P<0.01). Adiponectin levels in FF were unexpectedly significantly lower than serum levels in each group (P<0.05),and the two levels were strongly correlated to each other(r=0.38,P<0.01). The expression of adiponectin mRNA was not detected by RT-PCR on GCs in the whole groups. The serum adiponectin levels were positively correlated with estradiol and progesterone levels (r=0.001, P<0.05; r=0.391, P<0.05), but negatively correlated with BMI (r= -0.869, P<0.001). The serum adiponectin levels were positively correlated with number of eggs , fertilized eggs and thickness of endometrium (r=0.390,P<0.05;r=0.444,P<0.05;r=0.565,P<0.01)in obese women. However, this phenomenon wasn't present in women with normal weight. It was further observed that serum adiponectin levels and thickness of endometrium in women who conceived were higher than those in women who did not conceive (P<0.05).Conclusions:The expression of adiponectin receptors mRNA was detected on human follicular luteinized granulosa cells, but the mRNA expression of adiponectin was not detected. The expression of adiponectin and its receptors is closely correlated with obesity. And adiponectin may be a useful marker,especially in obese women, for early prediction of good IVF outcome.PartⅡExpression of adiponectin and its receptors in women with polycystic ovarian syndrome during IVF cyclesObjective: To compare adiponectin levels in serum and follicular fluid (FF) and the expression of mRNA of adiponectin and its receptors (AdipoR1/AdipoR2) in follicular luteinized granulosa cells (GCs) from women with polycystic ovarian syndrome (PCOS) and control group. And also, to investigate the relationship between adiponectin or its receptors levels and obesity, follicular maturation and the outcome of IVF-ET.Methods: Serum and FF were obtained from 71 women during the oocyte retrieval. These cases were divided into 4 subgroups: group 1, 14 women with PCOS and body mass index (BMI)>24kg/m2; group 2, 22 women with PCOS and BMI≤24kg/m2; group 3, 17 non-PCOS women with BMI>24kg/m2; group 4, 18 non-PCOS women with BMI≤24kg/m2. The adiponectin concentration was determined by ELISA, Sex hormone levels were by chemiluminescent immunoassay. The mRNA expression of adiponectin and its receptors were measured by RT-PCR.Results: There were no significant differences in either serum or follicular adiponectin levels or mRNA expression of adiponectin receptors on GCs between the PCOS groups and BMI-matched non-PCOS groups. However, serum adiponectin levels and expression of its receptors were significantly lower in obese women, either with PCOS or not, than in women with normal weight (P<0.05). The serum adiponectin levels were positively correlated with number of follicles whose diameters were larger than 14mm on HCG day (r=-0.394,P<0.05), but negatively correlated with BMI (r= -0.394, P<0.05) in women with PCOS. It was further observed that serum adiponectin levels in women who conceived were higher than those in women who did not (P<0.05).Conclusions: The expression of adiponectin and its receptors is closely correlated with obesity, but probably dose not play determining role in etiopathogenisis of women with PCOS. Adiponectin may be a useful marker for early prediction of good IVF outcome.