| Objective: 1. Observe the relationship of blood lead levels between children with bronchial asthma and normal children; 2. Observe the relationship of serum IL-4 levels between children with bronchial asthma and normal children; 3. Observe the relationship of serum IgE levels between children with bronchial asthma and normal children; 4. Investigate the relationship between the blood lead levels and serum IL-4, IgE in children with bronchial asthma.Methods: 45 cases of children with bronchial asthma were collected in clinic service and the ward of pediatrics in 1st affiliated hospital of Dalian Medical University, 30 cases of normal children were collected in clinic service from february 2008 to february 2009. Zeeman Effect of graphite furnace atomic absorption spectrometry was used to detect the blood Lead level, Radioimmunoassay and powder color Turbidimetry was used to detect serum IL-4 and IgE level, at the same time, Peripheral blood EOS level. Correlation was analyzed between blood lead, serum IL-4 and IgE in bronchial asthma children. Experimental dates were represented by x±s after test of normality. Sex composition was analyzed by chi square test, while age distribution was by analyzed t test. Mean between groups were compared by t test. Associativity between groups was analyzed by linar correlation. Significant differences were existed when P<0.05.Results:1. Serum Lead level:There was significant differences of blood lead between bronchial asthma 105.64±30.32ug/L and control groups 55.42±20.80ug/L, P<0.01. The blood lead of bronchial asthma was significantly higher than control group. 2. Serum IL-4 level: There were significant differences of blood IL-4 level between bronchial asthma 1.22±0.82ng/ml and control group 0.48±0.21ng/ml, P<0.01. The blood IL-4 of bronchial asthma was signifycantly higher than control group.3. Serum IgE level: There were significant differences of blood IL-4 level between bronchial asthma 264.53±62.501IU/ml and control group 83.49±26.72IU/ml, P<0.01 The blood IgE of bronchial asthma was significantly higher than control group.4. Peripheral blood EOS level: There were significant differences of blood EOS level between bronchial asthma 0.40±0.20×109/L and control group 0.20±0.10×10~9/L, P<0.01 The blood EOS of bronchial asthma was significantly higher than control group.5. There are positive relationship between blood lead and serum IL-4 r=0.469 p <0.001; There are positive relationship between blood lead and IgE r=0.570,p <0.001.Conclusions:1. The existence of immune disorders in children with asthma, IL-4, IgE and EOS were increased, which was closed with the occurrence and development of asthma-related. Its mechanism was closed with T-lympho- cyte in asthmatic children present Th2-like factor.2. Increased lead can damage the structure of bronchial mucosa, which cause chronic inflammation and bronchial hyperresponsiveness; At the same time, a wide range of toxic effects of lead can affect the function of the body defense system, in particular, it can inhibit T lymphocyte function, which lead to imbalance function between Th1 cell and Th2 cell. In the pathogenesis of children with bronchial asthma, increased blood lead can increase the level of serum IL-4 levels and serum IgE levels, which lead to the increased incidence of children bronchial with asthma increased, pollution of lead was one important induced factor of asthma. Therefore, lower the blood lead level provides theoretical basis for prevention and adjuvant therapy in children with bronchial asthma. |
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