Effects Of BFGF On Angiogenesis And MHC Gene Expression After Acute Myocardial Infarction In Rats With Type 2 Diabetes Mellitus

Objective: To evaluate the effect of intramyocardial administration of basic fibroblast growth factor(bFGF) on angiogenesis,infarct size(IS) and MHC mRNA after acute myocardial infarction(AMI) in rats with type 2 diabetes mellitus.Methods: GK rats and Wistar rats underwent left thoracotomy followed by the ligation of left anterior descending coronary artery. After model reproducing, the GK rats and Wistar rats were randomized to dissolvent control group and therapy group, and meanwhile erected sham group. Dissolvent control group and therapy group were received a directly intramyocardial injection of either PBS or bFGF, and sham group were also injected PBS in the same parts. After 3 weeks of breeding, the angiogenesis,IS and expression of non-infarcted myocardial MHC mRNA were assessed.Results:①Changes of electrocardiogram testify that the model of AMI is successful.②There was a significant increase in the density of capillaries and arterioles in bFGF therapy group (P<0.01), but the density increment of DM group were lower than NDM group (P<0.01).③The ischemic size of bFGF therapy group were decreased significantly compared with dissolvent control group (P<0.01). The ischemic size of DM group were increased significantly compared with NDM group (P<0.05).④Compared with sham group,β-MHC mRNA were all significantly increased in dissolvent control group, while a-MHC mRNA were all significantly decreased (P<0.01). In comparison with dissolvent control group,β-MHC mRNA were all significantly decreased, while a-MHC mRNA were all significantly increased in bFGF therapy group (P<0.01). Compared with NDM group, β-MHC mRNA were all significantly increased in DM group, while a-MHC mRNA were all significantly decreased (P<0.01).Conclusion:①Directly intramyocardial injection of bFGF could promote angiogenesis and reduce the ischemic size in ischemic myocardium, it played a role of protection for heart of myocardial infarction in diabetic rat.②bFGF could promote the expression of a-MHC mRNA and restrain the expression ofβ-MHC mRNA, it had beneficial effects on reversing LV myocardial pathologic switching of MHC isoform.③Protection of bFGF for heart of myocardial infarction were attenuated in rats with type 2 diabetes mellitus.