The Regulation Of ERK Signaling Pathway In Histamine-induced Rat Airway Smooth Muscle Cell Proliferation

BackgroundThis work was designed to explore the regulation of extracellular signal-regulated kinase (ERK) signaling pathway in histamine-induced rat airway smooth muscle cell proliferation.ObjectiveASMCs were isolated and cultured in vitro and then exposed to different doses of histanmine (Hist) and PD98059. The proliferative effect of ASMCs was measured using MTT microcolorimetric assay. The expression of ERK1/2 and phosphorylated ERK1/2 proteins were detected by western blot. ELISA was used to evaluate the levels of NF-κB in ASMCs, and then compared with each group.MethodsASMCs were isolated and cultured in vitro and then exposed to different doses of histanmine (Hist) and PD98059. The proliferative effect of ASMCs was measured using MTT microcolorimetric assay. The expression of ERK1/2 and phosphorylated ERK1/2 proteins were detected by western blot. ELISA was used to evaluate the levels of NF-κB in ASMCs, and then compared with each group. ResultsLow and high concentration of Hist were able to stimulate varying degrees of ASMCs proliferation, 100μmol / L when the cell survival rate reached 201.3%. Compared with control group, ASMCs proliferation in Hist group was increased significantly, while Hist + PD980059 group was lower than the latter one. The expression of p-ERK1/2 protein in Hist group increased markedly than the control group.Joined with PD980059, the expression of actived ERK1/2 in Hist group dropped significantly. The absorbance value in Hist group was higher than the control group, while Hist + PD98059 group was lower than the former one.ConclusionsHistanmine can induce proliferation of ASMCs significantly, not only did ERK signal channel play an important role in regulating histamine-induced rat ASMCs proliferation, but also may be involved in the Hist-induced NF-κB activation.