Determination Of Baicalin And Chlorogenic Acid In Traditional Chinese Medicine Patent Prescription By HPLC With Microwave-assisted Extraction

Traditional Chinese Medicine Patent Prescription(TCMPP) is a prescription which is produced in accordance with the provisions of the prescription,production processes and quality standards by Chinese herbal medicine under the guidence of Traditional Chinese Medicine(TCM) theory. TCMPP has many unique advantages,such as convenient to carry,easy to use, little toxicity and so on.With the modernization of TCM,TCMPP play more and more important role in the field of TCM.Baicalin and Chlorogenic acid exist in many kinds of plants.They can be used as bioactive compounds in lots of TCMPP.It is reported that Baicalin and Chlorogenic acid have shown antibacterial,anti-inflammatory,significant scavenging of free oxygen radicals and antioxidative activity.The microwave-assisted extraction of Baicalin and Chlorogenic acid of Yinhuang Tablets and Xiaoer Qingrening Granule was studied in this thesis.Determination of Baicalin and Chlorogenic acid from Yinhuang Tablets by microwave-assisted extraction(MAE) and RP-HPLC was reported in the first part of thesis.The effects of solvent concentration,microwave power, extracting time and extracting temperature were evaluated by single factor and orthogonal experiments.The extraction conditions of Baicalin and Chlorogenic acid from Yinhuang Tablets by microwave-assisted method were optimized as follows:the ethanol concentration was 70%;the microwave power was 255 W;the extracting time was 1 min and extracting temperature was 80℃.Under the optimized conditions,the recovery of Baicalin and Chlorogenic acid were 96.2%～100.3%and 96.5%～106.3%,respectively.The relative standard deviation of Baicalin and Chlorogenic acid were 3.69%(n=5) and 4.03%(n=5),respectively.Determination of Baicalin from Xiaoer Qingrening Granule by microwave-assisted extraction and RP-HPLC was reported in the second part of thesis.The effects of solvent concentration,microwave power,extracting time and extracting temperature were evaluated by orthogonal experiments. The extraction conditions of Baicalin from Xiaoer Qingrening Granule by microwave-assisted method were optimized as follows:the ethanol concentration was 70%;the microwave power was 255 W;the extracting time was 1 min and extracting temperature was 80℃.Under the optimized conditions,the recovery and the relative standard deviation of Baicalin were 96.9%～100.3%and 2.42%(n=6),respectively.Compared with ultrasound-assisted extraction and microwave-assisted extraction,MAE was simple and accurate with good reproducibility.The results show that microwave-assisted extraction-RP-HPLC is a stable and reliable method for the determination of the content of Baicalin and Chlorogenic acid in TCMPP,and it can be used as a referrence for quality control of Chlorogenic acid and Baicalin in TCMPP.