Expression Of Chemokine Receptor CCR5, CCR7 And CXCR4 In Endometriosis

Objective: We investigated the relationship between the production and development of endometriosis and three chemokine receptors—CCR5,CCR7 and CXCR4,in order to lay a solid basis for futher illustration of the cause of endometriosis and improvement of its present ways of diagnosis and cure.Methods: Taking normal endometrium without endometriosis as the experimental contral group, we focused on the study of eutopic and ectopic endometrium groups. Meanwhile, we classified above three groups respectively into proliferative phase and secretory phase according to the change of menstrual cycle again. RT-PCR was used to detect the expression of three chemokine receptors in normal,eupotic and ectopic endometrial tissues of women from mRNA level. According the result of it,we choosed CCR5 and CXCR4 as the objects of immunohistochemistry in order to detect the expression of their protein. Results: According to the analysis of SPSS 13.0, results were showed below when the mean difference is significant at the 0.05 level.In normal endometrium samples,some of them were able to transcribe CCR5 and others expressed few of it or even none of it.However,CCR5 was universally significantly transcribed in each eutopic endometrium and endometriotic foci sample. CCR5 in eutopic endometrium expressed higher than that in normal endometrium,but lower than that in endometriotic foic.Furthermore,in endometriotic foci tissue, CCR5 expressed in proliferative phase was lower than that in secretory phase.However, there was no evident difference of transcription of CCR5 between secretory phase and proliferative phase in normal endometrium.Additionally,the result of immunohistochemistry revealed that CCR5 presents negative in interstitial cells.However, the change of protein of CCR5 was same with its mRNA in the epidemic cells.CCR7 was universally transcribed in all tissues. CCR7 in eutopic endometrium expressed higher than that in endometriotic foic,but lower than that in normal endometrium.Furthermore, in normal endometrium tissue, CCR7 expressed in secretory phase was more than that in proliferative phase. However,there was no evident difference between secretory phase and proliferative phase in endometriotic foic. Due to the very small differences in above groups,the futher research still be needed.Most of normal endometrium samples were not able to transcribe CXCR4. However,CXCR4 was universally transcribed in each eutopic endometrium and endometriotic foci sample. CXCR4 in endometriotic foic expressed higher than that in normal endometrium,but lower than that in eutopic endometrium. Futhermore, there was no evident difference of transcription of CXCR4 between secretory phase and proliferative phase in endometriotic foic and normal endometrium. Additionally, The result of immunohistochemistry revealed that CXCR4 presents negative in those interstitial cells.However, the change of protein of CXCR4 is same with its mRNA in the epidemic cells.Conclusion:CCR5 and CXCR4 in endometriotic foci and eutopic endometrium were significantly higher than those in normal endometrium.It was safe to draw a conclusion that eutopic endometrium was more similar to ectopic endometrium other than normal endometrium in the expression of these chemokines receptors. CCR5 and CCR7 partly showed differences of mRNA transcription between proliferative phase and secretory phase.