Research Of The Effect Of Cytochrome P450 In Seven Prescriptions On The Basis Of Pinellia Tuber Couplet Medicines

The compatibility is the main clinical application forms of traditional chinese medicine,which is not only the feature and dominance of traditional chinese medicine,but also the emphasis and nodus of this field of science research.As an important phase I drug-metabolizing enzyme system in the human body,cytochrome P450 enzymes has significant value during the study of drug metabolism and drug-drug interaction.Therefore,it has far-reaching significance in researching the connotation of compatibility,as well as inheriting and developing the theory of traditional chinese medicine by analyzing the induction or inhibition effect of CYP450 in different compatibility of traditional chinese medicine.Through investigated on ancient literature and modern clinical experience, systematically sorted out and interpreted six kinds of medicinal compatibility, including the mutual reinforcement,mutual assistant,mutual detoxication,mutual restraint,mutual inhibition and antagonism,belonged to the core part of the theory of medicinal compatibility,namely the seven prescriptions.Meanwhile,taking the commonly used couplet medicines(pinellia tuber-ginger,pinellia tuber-aconite, pinellia tuber-golden thread,pinellia tuberliquorice root) as examples,expounded and explored the connotation of seven prescriptions,integrating property and flavor, meridian distribution,lifting,lowering,floating and sinking,and clinical experience,which belonged to traditional Chinese medicine fundamental theory.in order to identify and expand it.Then,through analyzed the significance of the metabolic enzyme CYP450 in the interaction of compatibility of Chinese Herbs,and summarized major functions of CYP1A2,CYP3A4 and CYP2E1 in transformation of drug,finally,we put forward to establish the research mode of compatibility of Chinese herbs on the basis of studying on the effect of CYP450's activity in different compatibility of Chinese herbs.The part of empirical study is on the basis of reseashing the four pairs of couplet medicines(pinellia tuber-ginger,pinellia tuber-aconite,pinellia tuber-golden thread, pinellia tuber-liquorice root) on the basis of studying on the effect of CYP450's activity in different compatibility of Chinese herbs by cocktail approach.Result is the level of CYP1A2 and CYP3A4 after received the aqueous extract of pinellia tuber -liquorice root was enhanced,but the level of CYP2E1 was not changed.But the other Chinese drug pairs groups have not effect the subtype enzymes of CYP450 either.So we can draw the conclusions from the directions of CYP450,that is pinellia tuber -liquorice root could induce CYP1A2 and CYP3A4,CYP2E1 was hardly affected.we presumed that liquorice root is the inductor of CYP1A2 and CYP3A4,so that it could promote the metabolism of pinellia tuber's toxicity compositions.this indicated that we can used this kind of compatibility mode under the circumstances of determine the treatment based on differentiation of symptoms and signs when utilizing the traditional Chinese medicine with toxicity.Pinellia tuber-aconite has no effect to the levis of subtype enzymes,which confirmed the debate of pinellia tuber and aconite have no relations of antagonism.Althouge the groups of pinellia tuber-ginger and pinellia tuber-golden thread could not induce or inhibite the activity of subtype enzymes,it still manifested that they would not effect the pharmacodynamic action of other drugs which mixed used during the clinical practice,but would not occur cumulation of toxicity compositions.These conclusions supplied rationale to the theory of "the the mutual reinforcement and mutual assistant should be used in the process of clinic ","utual detoxication and mutual restraint can used in the process of clinic",furthmore,provided for references in order to ues durg securely and rationally.