Research Of Relevance Between Plasma Endothelin, Nitric Oxide Levels And Periodontitis

ObjectiveEndothelial cells can produce immunoreactive substances, such as endothelin(ET), nitric oxide(NO), which regulate vascular tension. ET has a strong and long-lasting contraction on the arteries, veins, capillaries. NO is a major relaxation of vascular material released by the endothelial cells and certain nerve cells in vivo. Endogenous NO may be lead to a result of vasodilation and regulation of blood flow and blood pressure, and inhibit platelet adhesion in endothelial cells and platelet aggregation. The research of these mechanisms has become a hot topic to reveal a factor in the incidence of certain diseases. A large number of studies of cardiovascular diseases found that smoking increased the level of plasma ET and NO significantly, thereby affecting the occurrence of cardiovascular diseases and the development process. The aim of this research is to study the differences of plasma endothelin and nitric oxide levels variation between periodontitis patients and periodontal healthy people, And clarify the influence of smoking on plasma ET and NO levels. Thus discuss the possible correlative factor of periodontitis.Methods60 patients of chronic periodontitis, whom come from Oral Medicine outpatient of Binzhou Medical College hospital, were choosed as the experimental group, which were divided into mildly, moderate, the specific weight according to the severity of periodontitis.35 persons of periodontal healthy as the control group. Each group is divided into smoking and non-smoking group. All objects of study informed consent and meet the following conditions:①Have no systematic diseases;②Have not taken the antibiotic and non-steroid-anti-inflammatory drugs in 3 months;③Have not taken periodontal therapy in 1 year;④Feminine not pregnancy. Chronic periodontitis patients' standards:①Periodontal pocket> 3mm, inflammation and bleeding on probing,②Clinical attachment loss> 1mm,③Alveolar horizontal absorption or vertical absorption. Indexing standards of periodontitis: attachment loss (AL) 1-2mm mild , 3-4mm moderate, 5mm or more the specific weight. 2ml fasting blood of each people was collected and placed respectively in the test tube with 7.5% EDTA disodium (30μl) and aprotinin (40μl), after mixing, centrifugation and separation, plasma was collected and preserved in -20℃. Re-dissolve the samples at room temperature before determination, Re-centrifugation, take supernatant for determination. Use homogeneous competitive radioimmunoassay to determinate plasma ET content in each group directly, Adopt Nitrate reductase method to determine plasma NO level. ET and NO levels in plasma of periodontitis patients in each group once again are detected 4 weeks later after the initial therapy.ResultsPlasma ET and NO levels in smoking of control group are higher than that in non-smoking (p<0.05). Plasma ET and NO levels in smoking of periodontitis are higher than that in non-smoking (p<0.01). Plasma ET and NO levels of periodontitis were significantly higher than that of the control group(p<0.05), the severity of periodontitis and plasma ET and NO levels were statistically correlated(r_ET=0.839,r_NO=0.819 , p<0.01). The plasma ET and NO levels of periodontitis in each group were significantly decreased after initial therapy compared with pre-therapy, the difference was significant statisticaIIy(p<0.01). The varieties of before and after initial therapy in clinical indicators showed that the varieties in smokers was significantly less than non-smokers, the difference has statistical significance(p<0.05). ConclusionPlasma ET and NO levels of chronic periodontitis patients was significantly higher than that of periodontal healthy. After initial therapy, plasma ET and NO levels of periodontitis were significantly decreased. The results revealed that the plasma ET and NO levels have a positive correlation with the severity of periodontitis. Plasma ET and NO levels of smokers were significantly higher than that of non-smokers. Smoking can significantly affect the plasma ET and NO levels, thus affect the occurrence and development of periodontitis. Smoking can significantly affect the initial therapy effect of periodontitis, thereby affecting the prognosis of periodontitis; Smoking is an important risk factors of periodontitis.