| Objective:Th17 cells,a novel subset of IL-17-secreting CD4~+T cells,enhances the proinflammatory responses and has been implicated in several inflammatory disorder such as autoimmunity disease.Evaluate the expression of IL-17A,IL-10 and IFN-γin patients with different forms of inflammatory bowel disease(IBD)-ulcerative colitis(UC) and Crohn's disease(CD)-during clinical remission phase and investigate their role in the onset and treatment of IBD,providing a new method of treating IBD.Methods:Tissue samples were obtained endoscopically from patients with ulcerative colitis(UC)(n=46),Crohn's disease(CD)(n=12),and normal colorectal tissues (n=20).Sutherland for UC and CDAI for CD are used to determine the patients who are during clinical remission phase.IL-17 expression was evaluated by a standard immunohistochemical procedure.Serum IL-17A,IL-10 and IFN-γlevels were determined by ELISA.Results:1.There were no IL-17A~+ cells in normal colonic mucosa,while a marked increase in IL-17A~+ cells was seen in the CD and UC patients.In UC patients, IL-17A~+ cells were located mainly within the lamina propria but in CD patients these cells were scattered throughout the submucosa and muscularis propria.2.IL-17A:The level of IL-17 significantly increased in IBD patients, 14.48±4.37pg/ml in UC groups,17.08±2.48pg/ml in CD groups,while it was not detected in the sera of normal individuals. 3.IL-10:IL-10 in patients with Crohn's disease was significantly higher than that in patients with ulcerative colitis and control group(P＜0.05 and P＜0.05, respectively),with 72.37±12.44pg/ml in CD groups,43.72±7.63pg/ml in UC groups, while 40.97±16.96pg/ml in control groups.4.IFN-γ:Serum IFN-γlevels are 28.58±2.41pg/ml in CD groups, 29.02±4.12pg/ml in UC groups,and 29.71±2.09pg/ml in control groups.The difference between them is nonsense.Conclusions:1.IL-10 is a kind of anti-inflammatory cytokines secreted by Th2 cells,which can inhibit the antigen presentation of macrophage and monocyte and down-regulate the secretion of IFN-γ.The up-regulation of IL-10 seems to be due to response to pro-inflammatory cytokine activity.It can down-regulate the activity of immuno and non-immuno cells,reduce the production of pro-inflammatory cytokine,relieve the progression of IBD.The serum level of IFN-γin control group equal with CD and UC groups may be related to the down-regulation of the pro-inflammatory cytokines as the result of therapy with 5-ASA preparations,which have the suppressive effect on the immune systems.2.The level of IL-17A significantly increased in IBD patients,while it was not detected in the sera of normal individuals,the same result is found in the stain with anti-IL-17A antibodies.The reason why 5-ASA as a maintenance therapy of IBD can not down-regulate the serum level of IL-17A may be that IL-23/IL-17 axis play an important role in infection of IBD,which is different from Th1 and Th2 pathways.There were no IL-17A~+ cells in normal colonic mucosa,while a marked increase in IL-17A~+ cells was seen in the CD and UC patients,hinting that the infiltrative lymph cells conclude Th17 cells.In UC patients,IL-17A~+ cells were located mainly within the lamina propria in UC patients but in CD patients these cells were scattered throughout the submucosa and muscularis propria.Senses:IL-17A has been found in serum and mucosa of IBD patients during clinical remission phase.Th17 effector pathway may contribute to inflammatory bowl disease's disease pathogenesis.Studies in IBD patients have confirmed the predominant role of the IL-23/IL-17 pathway,indicating that this could represent an important future therapeutic target. Objective:To investigate the expression of Slit2 and its correlation with microvessel density in human esophageal squamous cell carcinomas and paracancerous tissues,and to analyze their connection to clinicopathological findings.Methods:Immunohistochemical staining of Slit2 and CD34 was performed with 64 cases of cancerous tissues and 36 cases of para-cancer tissues.To detect the relationship between the expression of Slit2,MVD and age,gender,Tumor size,histology, invasion depth.TNM stage,lymph node metastasis and distant metastasis.All the data for statistics using spss11.5.Results:The positive rates of Slit2 in cancerous tissues and para-cancer tissues were respectively 70.3%and 16.7%,the difference between them had statistical significance (χ~2=26.53,P＜0.01).The MVD in cancerous tissues was significantly higher in comparison to para-cancer tissues(P＜0.01).The MVD in the tumor tissue with positive expression of Slit2 was much higher than that in the nagetive one(P＜0.05). The expression rate of Slit2 and the enumeration of MVD were significantly associated with tumor size,invasion depth,TNM stage and lymph node metastasis. The expression of Slit2 was positively correlated with the MVD(r=0.683,P＜0.01).Conclusions:These results suggest that Slit2 expression and enumeration of MVD is associated with clinicopathological findings of esophageal squamous cell carcinomas.Slit2 may play an important role in tumor angiogenesis of esophageal squamous cell. |
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