The Effect And Mechanism Of BuYangHuanWuDecoction(BYHWD) By IL-1 System And Caspase Express After Cerebral Ischemia-Reperfusion In Rats

Objective:To investigate the effect of the four kinds of active fraction of BuYangHuanWuDecoction(BYHWD) by IL-1 system,and the mechanism of active fraction of BYHWD by adjusting caspase-1,3,8 expression after cerebral ischemia-reperfusion.Further to expound the material base of BYHWD antagonising cerebral ischemia and the mechanism of BYHWD inhibiting inflammatory reaction,antagonising intercellular adhesion and neuronic apoptosis after cerebral ischemia.Methods:Adopt the rat middle cerebral artery occlusion(MCAO)method to induce cerebral ischemia-reperfusion model.Rats of SD are randomly divided into 7 groups:sham operation,model group,alkaloid group(104.54mg/kg), glycoside group(168.5mg/kg),polysaccharide group(405mg/kg),aglycone group(16.5mg/kg) and nimodiping group(1mg/kg).Polysaccharide group and aglycone group were given medcine in 1 hour before ischemia,the other groups were given medcine in 5 min before ischemia,sham operation and model group were given equal volume normal saline.The expression of protein IL-1β, ICAM-1 and Caspase-1,3,8 were observed by immunochemistry after cerebral ischemia for 2 hours followed by reperfusion for 46 hours.Result:1.After cerebral ischemia for 2 hours followed by reperfusion for 46 hours,IL-1βpositive reactions increased obviously in the hippocampi,cortex and medulla,alkaloid could inhibit IL-1βprotein express,but nimodipping couldn't inhibit IL-1βprotein express.2.After cerebral ischemia for 2 hours followed by reperfusion for 46 hours,ICAM-1 positive reactions increased obviously in the hippocampi and cortex,glycoside could inhibit ICAM-1 protein express of the cortex,nimodiping could inhibit ICAM-1 protein express of the hippocampi。3.After cerebral ischemia for 2 hours followed by reperfusion for 46 hours,caspase-1 positive reaction increased obviously in the choroid,alkaloid,glycoside,polysaccharide,and aglycone could inhibit caspase-1 protein express,but nimodipping couldn't inhibit caspase-1 protein express.4.After cerebral ischemia for 2 hours followed by reperfusion for 46 hours,caspase -3 positive reactions increased obviously in the hippocampi,cortex and medulla,Alkaloid,glycoside,aglycone and nimodiping could inhibit caspase-3 protein express,but polysaccharide couldn't inhibit caspase-3 protein express.5.After cerebral ischemia for 2 hours followed by reperfusion for 46 hours,caspase-8 positive reactions increased a little in the hippocampi,cortex and medulla,only glycoside could decrease caspase-8 protein express. Conclusions:The four kinds of active fraction of BuyangHuanWuDecoction (BYHWD) could antagonize cerebral ischemia injury.Alkaliod could inhibit IL-1βprotein express,glycoside could inhibit ICAM-1 protein express,alkaloid,glycoside polysaccharide and aglycone could inhibit caspase-1 protein express, alkaloid,glycoside and aglycone could inhibit caspase-3 protein express.The research reveal alkaloid,glycoside,polysaccharide and aglycone could be the important material base of BYHWD antagonising cerebral ischemia.The mechanism of BYHWD could be associated with inhibiting inflammatory reaction and antagonising intercellular adhesion,soakage and neuronic apoptosis after cerebral ischemia.