A Study For The Expressions Of C-Myc And GSTs In Primary Hepatic Carcinoma

Objective To investigate the expressions of c-Myc and glutathione S-transferase (GSTs) in Primary Hepatic Carcinoma (PHC) in order to explore the correlation between themselves and their relationship to the occurrence of Primary Hepatic Carcinoma (PHC).Methods The specimens from 47 patients with PHC and 20 normal livers were fixed in 10% formalin and routinely embedded in paraffin. The specimens were continuously sliced into 4μm-thick sections. Envision^TM immunohistochemistry was performed to detect the expressions of c-Myc and GSTs with monoclonal tag antibodies and scored them under high-power microscopy.Results The expressive positive rates of c-Myc and GSTs in cancer tissues are significantly higher than those in the normal hepatic tissues: 44.7% vs 5.0%, 59.6% vs 5.0% (P=0.002, P<0.001); The expressive positive rate of c-Myc in low differentiation cancer tissue is significantly higher than that in the high differentiation cancer tissue ( 61.9% vs 30.8%, P=0.033); The expression of GSTs in high differentiation cancer group is significantly higher than that in the low differentiation cancer group (84.6% vs 28.6%, P<0.001); The expressive positive rate of GSTs protein in cancer tissues with Hepatitis B (67.5%) is significantly higher than that in cancer tissues without Hepatitis B (16.7%, P=0.028); There is no obvious relationship between the expressions of c-Myc and GSTs in cancer tissues of PHC and other main clinicopathological characteristics of PHC (P>0.05). There is no evident correlation between the expressive scores of c-Myc and that of GSTs in cancer tissues of PHC (r=-0.220, P>0.05).Conclusion The over expressions of c-Myc and GSTs are closely related to the occurrence, developing processs and differentiation of Primary Hepatic Carcinoma (PHC). The expression of GSTs is related to the infection of Hepatitis B Virus (HBV) in PHC. There is no evident correlation between the expressions of c-Myc and GSTs in PHC.