Study On The Inhibitory Action Of Cisplatin On Choriocarcinoma Cell Lines In Vitro

Background: choriocarcinoma is malignant trophoblastic tumor, which is sensitive to chemotherapy. 5-fluorouracil (5-FU) single or combined with other anti-tumor drugs is the capital chemotherapy, but the effective dose of 5-FU closes to toxic dose, so serious toxicity usually occurs in clinical.Aims: Measure the survival ratio, inhibition ratio and apoptosis ratio in choriocarcinoma cell lines JAR and JEG-3 cultured in vitro after using different concentrations of cisplatin(DDP) , and compare with 5-FU to provide primary clues of laboratory for curing choriocarcinoma using DDP.Methods: Choriocarcinoma cell lines JAR and JEG-3 cultured in vitro were used in study. JAR and JEG-3 cell lines were treated respectively in five concentrations of DDP or 5-FU for 24 hours, 48 hours and 72 hours. Then optical density (OD), which was an indirect response to cell survival and inhibition ratio, was detected by micro plate reader. Calculate the IC50 via the method of Bliss. Then two kinds of cells were cultured in the different drugs that concentration was IC50 after 48 hours apoptosis ratio of different cells was detected by flow cytometry.Results: (1) DDP could inhibit generation of JAR and JEG-3 (p< 0.05),and the total inhibitory action caused by DDP and 5-FU showed no significant difference (p>0.05).(2) DDP group showed strong positive correlation between inhibition ratio and concentrations in both JAR cell line and JEG-3 cell line(r_JAR= 0.814, r_JEG-3 = 0.823, p<0.05). (3) DDP group showed moderate-intensity positive correlation between inhibition ratio and time in both JAR cell line and JEG-3 cell line (r_JAR = 0.472, T_JEG-3 = 0.684, p <0.05). (4) The inhibition effect in JAR cell line caused by DDP with concentration of 25mg / L is stronger than that in JEG-3 cell line (p < 0.05), while the inhibition effect caused by corresponding concentration of 5-FU (250mg / L) in JEG-3 cell line is stronger than that in JAR cell line (p <0.05); (5) Both JAR cell line and JEG-3 cell line could be induced to apoptosis or necrosis by DDP. The apoptosis ratio of JAR was 32.33±1.51%, and that of JEG-3 was 32.57±0.48%. The necrosis ratio of JAR was 17.24±0.48%, and that of JEG-3 was 17.32±0.51%. The total apoptosis ratio induced by DDP or 5-FU in both JAR cell lines and JEG-3 cell lines was no significant different (p>0.05), but the necrosis ratio of JAR cell line caused by DDP was lower than 5-FU.Conclusions: (1) DDP could inhibit generation of JAR and JEG-3 cell line, and inhibition effects were the same as 5-FU, addition, the inhibition effects were correlation with both does and time. (2)The inhibition effect of JAR cell line caused by DDP in certain concentration (25mg/L) was stronger than JEG-3 cell line, and the inhibition effect of JEG-3 cell line caused by 5-FU in certain concentration (250mg/L) was stronger than JAR cell line. (3) The apoptosis effects induced by DDP and 5-FU were almost the same in JAR cell line and JEG-3 cell line.