| Objective To investigate the effects of 4℃HTS in rat brain tissues after SCA and resuscitation .Methods 54 Sprague-Dawley male rats were randomly divided into two groups:the first group included:shame-operated(A) group,NS-treated(B) group,4℃NS-treated(C) group,HTS-treated (D)group, 4℃HTS-treated (D) group.Each group included 6 tats. Except group A,the rest groups were administered at the onset of CPR. The second group included: NS-treated(B1) group at the 30 minutes after ROSC, NS-treated(B2) group at the 60 minutes after ROSC, 4℃HTS-treated(E1) group at the 30 minutes after ROSC, 4℃HTS-treated(E2) group at the 60 minutes after ROSC. Each group was administered in 4ml/kg.The concentration of HTS was 7.5%.SCA was induced on rats by asphyxia. We had a continuous monitoring on the change of MAP in 60 minutes after administration. We also determined the concentration of blood serum Na+ in each group at 60 minutes after administration,and then we calculated plasma osmotic pressure in each group. We recorded NDS at 6h,12h and 24h after ROSC. At 24h after ROSC, we got the venous blood to detcect the concentration of serum S100 protein in each group,and sacrificed the rats, got the brain tissues to compare the expression of caspase-3 ,GFAP and myelin staining in hippocampus, and calculated the brain water content.Statistical method: Comparison the mean of multiple samples used Student-Keuls(SNK-q).Results The first group: There was no statistical difference in the change of MAP and Na+, plasma osmotic pressure in 60 minutes after administered in each group(P>0.05). Compared with NS-treated group, NDS in 4℃HTS-treated group at 6h,12h,24h after ROSC increased (P<0.05);that in 4℃NS-treated group and HTS-treated group was no statistical difference(P>0.05); also,there was no statistical difference among 4℃NS-treated group,HTS-treated group and 4℃HTS-treated group(P>0.05). Compared with group shame-operated group, the concentration of serum S100 protein in the rest group was much higher (P<0.01); There was statistical difference among NS-treated group, 4℃NS-treated group, HTS-treated group and 4℃HTS-treated group(P<0.01 or P<0.05). Compared with group shame-operated group, the expression of caspase-3 and GFAP among NS-treated group, 4℃NS-treated group and HTS-treated group was much more(P<0.01), that in group 4℃HTS-treated group was no statistical difference(P>0.05); There was statistical difference among NS-treated group, 4℃NS-treated group, HTS-treated group and 4℃HTS-treated group ( P<0.01or P<0.05).Compared with shame-operated group, the brain water content among NS-treated group, 4℃NS-treated group, HTS-treated group and 4℃HTS-treated group was much higher(P<0.01); Compared with NS-treated group, there was statistical difference among 4℃NS-treated group,HTS-treated group and 4℃HTS-treated group(P<0.01 or P<0.05);there was no statistical difference in the brain water content among 4℃NS-treated group, HTS-treated group and 4℃HTS-treated group (P>0.05). There was no statistical difference in numbers of myelin in each group(P>0.05). The second group: there was no statistical difference in the change of MAP and Na+, plasma osmotic pressure in 60 minutes after administered in each group(P>0.05). Compared with NS-treated group,NS-treated group at the 30 minutes after ROSC and NS-treated group at the 60 minutes after ROSC, NDS in group 4℃HTS-treated group, 4℃HTS-treated group at the 30 minutes after ROSC and 4℃HTS-treated group at the 60 minutes after ROSC at 6h,12h,24h after ROSC increased (P<0.05), but there was no statistical difference among 4℃HTS-treated group, 4℃HTS-treated group at the 30 minutes after ROSC and 4℃HTS-treated group at the 60 minutes after ROSC (P>0.05). Compared with NS-treated group,NS-treated group at the 30 minutes after ROSC and NS-treated group at the 60 minutes after ROSC , the concentration of serum S100 protein, the expression of caspase-3 and GFAP and the brain water content in group 4℃HTS-treated group, 4℃HTS-treated group at the 30 minutes after ROSC and 4℃HTS-treated group at the 60 minutes after ROSC at 24h after were much lower(P<0.01), but there was no statistical difference among 4℃HTS-treated group, 4℃HTS-treated group at the 30 minutes after ROSC and 4℃HTS-treated group at the 60 minutes after ROSC (P>0.05). There was no statistical difference in numbers of myelin in each group(P>0.05).Conclusion 4℃HTS-treated after SCA can raise and NDS, decrease serum S100 protein level and the expression of caspase-3 and GFAP in hippocampus, reduce cerebral edema and histopathological damages,protect the brain tissius. There was no significant effect on serum Na+ and osmotic pressure as well as myelin in brain tissues after 4℃HTS treated. 4℃HTS-treated at the onset of CPR,at the 30 minutes after ROSC, at the 60 minutes after ROSC can all pretect brain tissues,and the effects were same. |
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