Early Empirical Study For The Treatment Of Rat Acute Hepatic Failure By Auxiliary Heterotopic Liver Transplantation In Spleen Fossa

Objective:To establish a stable rat model of acute hepatic failure and a rat model of heterotopic auxiliary liver transplantation in spleen fossa with splenectomy.To observate the survival rate.To study the pristine effects of graft liver in supporting acute hepatic failure.To investigate the relationship between the regeneration of host liver and the atrophy of graft liver.Methods:Healthy SD rats (unlimiting female and male) undergoing 85% partial (left lateral lobe,left medial lobe,middle lobe,right up lobe)hepatectomy,divide it randomly into two groups as follows: group A(n=40) after a left nephrectomy and lienectomy,the graft liver which was reduced to about 30% of the original size,was inplanted into the spleen fossa of the recipient,s abdomen.While A1(n=20) for the observation of survival rates,and A2(n=20) for the collection of blood and liver preparation.Group B(n=20) control group with a left nephrectomy and lienectomy.Ob servating the survival rate of groupA1 and B;Detecting the blood-serum of group A2 (1d,3d,7d,14d after operation) and group B (20h,14d after operation).Executed the suvival rat of group A2(1d,7d,14d after operation) and group B(20h,14d after operation), take out of graft liver and host liver for weighing and hematoxylin and eosin staining.Results:Survival Rate During the experiment,the 24h postoperative survival rate of group B is 20% ,while 3d,7d,14d postoperative survival rates are 10%.meanwhile,the suvival rates of group A1 at 1d,3d,7d,14d postoperative are 90%,75%,65%,65%.The homochronous survival rate of group A1 is highter than group B obviously (P<0.05). Blood-serum Detection The parameter of ALT,AST,GGT,TB,PT in rats of group B rising sharply at 20h after operation,and it was still at a hight level; The index of group A2 degraded obviously and approach to the nomal value gradually with time variancing after operation. The homochronous index of group A2 is more less than group B(P<0.05). Histopathology The weight of host liver increased gradually with time variancing after operation,while it,s no significant change 7d postoperative and lighten slightly 14d postoperative.1d postoperative ,the host liver start swelling and show hyperplasia under microscope; While the graft liver show no significant change under macroscopic and microscope observation.7d postoperative, the host liver become more hypertrophy than 1d postoperative,and change in bloom.Under microscope, there are conjugate nuclei in some hepatic cells,which indicated hyperplasia;Meanwhile,the graft liver still have no obvious change except few lymphocytes imbibition in portal area. 14d postoperative,the host liver enlarge its volume significantly and show hyperplasia briskly under microscope; At the same time,the volume of graft liver decreased slightly.Under microscope, hepatic lobule almost nomal,hepatic cells show slight degeneration and some necrosis, there are monocytes and lymphocytes imbibition in portal area,and the connective tissue also show hyperplasia in extent.Conclusions:Undergoing 85% partial hepatectomy is an scientific and advisable method to develop acute hepatic failure in rat, it is also stable and repeatable;A -uxiliary heterotopic liver transplantion in spleen fossa with splenectomy is feasable and scientific;The graft liver play an exact support role for the rat on passing the acute hepatic failure time;The host liver is weighting and hyperplasing gradually with time variancing after operation, the graft liver show no significant change under macroscopic and microscope observation at prophase,but it show slight degeneration with the recovering of host liver.