| Chronic osteomyelitis is a common orthopedic disease,75% of which is caused by Staphylococcus aureus Rosenbach.We often need to do bone grafting to promote bone defects healing for the chronic osteomyelitis patients.A good bone filling material must not only fill the bone defects but also kill residual pathogens. Many scholars at home and abroad have studied composite bone repairing materials mixed with antibiotics (ie antibiotic delivery system) to repair bone defects created by infection in the primary healing period and they have made significant progress. However, compared with natural bone ,there is much to be desired.Therefore,it is urgent to develop a bioactive implant material which has both good biocompatibility and high efficiency of anti-bacterial nature.Recently, extensive attention have been paid to the inorganic nano-antibacterial materials for its high efficiency of anti-bacterial nature, higher security and stability .ObjectiveTo prepare the TiO2-Ag-nHA/PA66 bone filler antibacterial bone filler nanophase materialsby co-polymerization method, and to research the antibacterial effect of this material, in vitro. Explore the feasibility of using this material to fill the bone defect after chronic osteomyelitis.This experiment has the following 2 parts: Part 1 Preparation and performance testing of nano-composite bone filling materials containing sliverPart 2 Antibacterial properties of nano-composite bone filling materials containing sliver in vitro.Method1 .Preparation and performance testing of nano-composite bone filling materials containing sliverThe antibacterial nano-composite bone filling materials containing sliver was prepared by co-polymerization method. The chemical components were characterized by X-ray Diffraction, Electron microscope, Atomic absorption spectrometry,et al.2. Antibacterial properties of nano-composite bone filling materials containing sliver in vitro.Bacterial adherence inhibition effect and antibacterial property of nano-composite bone filling materials containing sliver to Staphylococcus aureus and Escherichia coli were tested by Agar plate diffusion method, The plate-counting method and SEM.Results1 .Preparation and performance testing of nano-composite bone filling materials containing sliverThe production and performance testing XRD of the Anti-bacterial nano-composite material bone filler with sliver showed that anti-bacterial silver nano-composite materials bone-filled contains HA, but because the Ag + and Ti02 is so low that both of them are not detected; and the SEM showed that anti-bacterial silver nano-composite materials bone filler is porous structure, with an average diameter of 100-500μm; Atomic absorption spectrometry showed light absorption potential anti-bacterial silver nano-composite material bone filler contain Ag, Ti, and the content of them is 0.64% and 2.35%.2. Antibacterial properties of nano-composite bone filling materials containing sliver in vitro.The diameter of bacterial inhibition zones of silver nano-hydroxyapatite composite material compared with n-HA/PA66. In Experimental group, the diameter of bacterial inhibition zones of both two test bacterial strains are the largest in the first day, followed by the diameter narrowing over time, culminating in Staphylococcus aureus ATCC25923 group sustained 33 days and in Bacillus coli ATCC25922 group sustained 21 days. In the control group,there are not bacterial inhibition zones of both two test bacterial strains;The bacteria colony assay results demonstrated that the silver nano-hydroxyapatite composite material for bone filling has antibacterial effect 94.8% of S.aureus and 86.2% of E. coli were killed.SEM showed obviously fewer Staphylococcus aureus and Bacillus coli on the surface of nano-composite bone filling materials containing sliver.Conclusion:nano-composite bone filling materials containing sliver prepared by co-polymerization method have superior antimicrobial activity in vitro. Depending on this,it is useful to pack bone defect in chronic osteomyelitis. |
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