Effect Of Alendronate On The Biological Characteristics Of Osteoblasts And Osteoclasts In Co-Culture System

Objective:â‘ To investigate the effect of Alendronate on Proliferation and the mRNA expression of OPG and RANKL of Human Osteoblasts.â‘¡To investigate the effect of Alendronate on the biological characteristics of osteoblasts and osteoclasts in co-culture system.Materials: The bone fragment were obtained from a surgical operation. Alendronate was presented by Merck Sharp &Dohme S.p.A. Collagenase-â…¡and Trypsin were obtained from Invitrog. FBS was obtained from cell scinence.Methods:â‘ Osteoblasts isolated from Human ilium cancellous bone were treated with various concentrations of Alendronate (10-9~10-4M).â‘¡To investigate the effect of the concentrations of Alendronate(10-8M) on the biological characteristics of osteoblasts and osteoclasts in co-culture system.â‘¢MMT method was used to assay proliferation of osteoblasts. The osteoblasts were plated at the concentrations of 10-9~10-5 M, seventy two hours later treatment factors were set. Total RAN was extracted from osteoblasts using TRIzol , and mRNA expression of OPG and RANKL were examined using RT-PCR.Results:â‘ 10-5,10-4M alendronate inhibited proliferation of osteoblast; The proliferation of osteoblasts increased under the stimulation of Alendronate range 10-9~10-6M (P < 0.05),Peak levels were gained at concentration of 10-8M. Alendronate concentration-dependently inhibited RANKL mRNA expression,10-5M Alendronate has a maximum effect after 72 h of treatment( P<0.01).Alendronate concentration-dependently increased OPG mRNA expression,10-6M Alendronate has a maximum effect after 72 h of treatment( P<0.01).But it is reduced on 10-5M.â‘¡The proliferation and the ALP activity of osteoblasts increased by Alendronate in co-culture system. The bone lacuna value and the TRAP activity of osteoclast decreased by Alendronate in co-culture system.Conclusion:â‘ Alendronate can increase the proliferation of osteoblast. The effect has a proper concentration of Alendronate. Alendronate can affect the bone metabolism by adjusting the OPG/RANKL mRNA expression.â‘¡Alendronate can increase the activity of osteoblast and decrease the activity of osteoclast.