Expression Of E-Selectin In Colorectal Neoplasm And Its Role In The Metastasis Of Blood Line

ObjectiveTo investigate the level of cell adhesion molecule E-selectin in colorectal neoplasm and its relations with metastasis in the blood line.Methods1 E-selectin expression in colorectal carcinoma and normal colorectal tissue were detected with immunohisochemical technique.2 Using the reverse transcriptase-polymerase chain reaction (RT-PCR),CK20mRNA expression was examined in peripheral blood.3 The change of shift ability of human colorectal neoplasm cell line was analyzed using the Transwell chamber.Results1 The expressing rate of E-selectin was 57.5%, obviously higher than 6.67% in the normal colorectal tissue(P<0.05).2 The positive rate of CK2OmRNA in peripheral blood of total 40 patients with colorectal carcinomas was 70%(28/40) ,obviously is higher than 6.67%(1/15) in healthy volunteers(P<0.05). 3 With the increase in cancentration of E-selectin (0μg/L,1μg/L,5μg/L,10μg/L),the number of intestine cancer cells penetranting the membrane increased large [(13.4±3.11), (27.2±4.13), (52.9±3.56), (100.7±8.15)](P<0.05).ConclusionE-selectin in the colorectal neoplasm assumed is highly expressed possibly and plays certain role in the metastasis of blood line.