| Objective: Acordding to the mechanism of the ischemia-reperfusion, lipid peroxidation and endotheliocyte injury To investigate the protection of PGE1 to acute ischemia-reperfusion and the relation of the activity dosage..Methods: The study was performed in 30 SD rats were divided into 5 groups at random: ischemia group(I) and ischemia-reperfusion groups (IR) including no medication group (IR0) and 3 medication groups (2ug/ml/kg , 4ug/ml/kg , 8 ug/ml/kg). Rats were ischemic for 120 minutes and IR1-3 groups were medicated 5 minutes before reperfusion. Blood samples were taken from femoral vein that 0.5h,2hand 4h after perfusion respectively.The meantime we take the femoral artery for pathology. After all we observed the change of ET-1,MDA and SOD in each group of pathology and electron microscope.Results: IR groups Compared with I groups ,the concentration of MDA increased (P<0.05),the activity of SOD declined(P<0.05),and the expression of ET-1 increased (P<0.01) . Medication groups contrast to IR0 group, the concentration of MDA reduced(P<0.05) , the activity of SOD increased (P<0.05) and the expression of ET-1 declined(P<0.001).The artery patheology of high dosage group,we find out that the platelet and leucocyte,et al hesion oto be decreased and. the mitochondrial swelling of endodermis to be improved in electron microscope.Conclusion:Lipid peroxidation is not obvious in rats 120mins of the ischemical.Lipo-PGE1 has the protection effect to ischemia-reperfusion of hind limb..PGE1 can reduce antioxidant ,protect endothelial cells and they were related to the dosage of drug. Futhermore,the results demonstrated the group of high dosage has obvious effects on limb ischima-reperfusion, Lipo-PGE1 can dilate the femoral artery which is important and the helpful for the clinical patients of lower extremity. |
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