| Objective: To isolate and cultivate ADSCs from rabbit fat tissue,observe their growth and passage;To establish scar mould at rabbit ear,search for feasibility of scar mould at rabbit ear;Explore the new method that ADSCs treat hyperplastic scar.Methods: Select six New-Zealand albino rabbits(all of them are male), The stem cells were isolated from the fold inguen adipose tissue of rabbit and cultured with collagenase digestion.Making use of inverted microscope undertake morphology observation everday;Established scar mould at rabbiy ear:design round wound of entry whose diameter is 1cm on rabbit auricular gaster side,then excise from skin to cartilage as well as perichondrium,then hematischesis and bandaging.There are four wound of entry each ear, the left is experiment group, and the right is control group;We use ADSCs cultured in chapter one which also grow actively 1ml to ring-shaped inject above cartilage layer of rabbit auricular hyperplastic scar in experiment group on post-operation 1d,7d,and 20d( 0.2ml each scar).We inject the same dose nomal saline to the control group.When hyperplasia is most conspicuous,we excise both hyperplastic scars,formalin fixation,paraffin imbedding ,slice and dyeing.Result: Cell proliferation is prosperity,fibroblast like,the cells primary cultured confluence 70%-80% in only 7-10 days.Conventional serial sub- cultivation can be progressed. Epithelization in scar mould will be finished 20d after operation, most obvious hyperplasty will take on about 30d,and the height will last about 2 months.Then part will emolliate,and about half continue to be hyperplasia state,the longest duration has exceeded 180 days; The experiment group has significant deviation from the control group statistically.Conclusion: The ADSCs of rabbits isolated in this trial are charac- terized by stable growth and quick proliferation in vitro. ADSCs have effect on rabbit auricular scar. |
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