Relationships Between Level Of Anti-egg Nucleus Antibody In Human Serum And HLA-DQB1 Allelic Polymorphism And The Immunological Characters And Clinical Significance Of The Antibody

Objective: Anti-egg nucleus antibody is specific antibody to egg nucleus antigen in human serum. This antibody can not only be found in patients with autoimmune disease but also in patients with gastric cancer. In this study, we detected the anti-egg nucleus antibody in random people and in different years of age group,gender group, and analyzed its distribution. Furthermore, we investigated the relationships between the antibody and HLA-DQB1 allelic genes. Finally we inquired its immunological characters and explored its clinical significance in ICU patients.Methods:1. Blood samples were collected from random population in Dalian Locomotive Hospital (51 male, 43 female with average age of 42.7). We used ELISA to detect the positive rate of anti-egg nucleus antibodies in serum.2. Study on the distribution of anti-egg nucleus antibody among people of different age and gender: Subjects were selected from the people who had passed the physical examination in the second Hospital Affiliated Dalian Medical University. It included 10 males and 10 females at the age over 70 (elderly), 10 males and 10 females at the age lower than 30 (young), respectively. They were allocated to elderly group and young group and also male group and female group, respectively.3. Screening of group with the maximum value of anti-egg nucleus antibody: The titer of serum anti-egg nucleus antibodies in 600 people who had passed the physical examination were detected by ELISA. The five people with the highest value (> x +SD) in each 100 people were selected to achieve a total number of 30. 100 people who had passed the physical examination were detected as control group. In order to investigate the relationships between anti-egg nucleus antibody and HLA-DQB1 allelo- morphic genes, the allelomorphic genes of HLA-DQB1 were amplified.4. Egg nucleic acid blocking test was used to study whether the anti-egg nucleus antibody combined with egg nucleic acid.5. Heterophile test was used to study whether the anti-egg nucleus antibody has heterophile property.6. 20 normal people in physical examinations of Dalian Locomotive Hospital (9 male, 11 female with average age of 49.2) were collected. The titer of anti-egg nucleus antibody,IgM and IgG blood group antibodies in serum were detected respectively.7. The serum anti-egg nucleus antibody of Kunming young mice fed with egg nucleus for 20 days was compared with the mice of control group were fed with normal mice food. It is to study the relationships between anti-egg nucleus antibody and food to explore the source of the antibody.8. Patients came from ICU of the second Hospital Affiliated Dalian Medical Hospital. All of the patients were satisfied with the diagnosis standards of ICU. They were divided into two groups according to the hospitalization standards: Patients with severe middle and late period of organ failure group (ICU chronic diseases group) and patients with acute disease group (ICU acute diseases group). ICU chronic diseases include leukemia, metastatic carcinoma and diabetes mellitus and so on; ICU acute diseases include trauma and acute massive hemorrhage and so on. ICU chronic diseases group contained 36 patients (14 males and 22 females with average age of 55.4) and ICU acute diseases group contained 20 patients (13 males and 7 females with average age of 52.5). 20 people who had passed the physical examination were measured as control group (9 males and 11 females with average age of 49.2). Among them, patients suffered from autoimmune diseases such as SLE and gastric cancer were excluded from both of acute diseases group and chronic diseases group. The purpose of this study is to find the distribution characters of the anti-egg nucleus antibody in patients of ICU and the clinical significance. Results:1. Positive rate of anti-egg nucleus antibody in random population was 100%.2. No significant differences of anti-egg nucleus antibody were found in groups with different age and gender respectively (P>0.05).3. Significant difference in HLA-DQB1*02 was confirmed between group with maximum value of anti-egg nucleus antibody and control group (P<0.05), the positive rate of HLA-DQB1*02 was 31.7% in maximum value group. No significant differences in the other subtypes were found.4. There was a significant difference between egg nucleic acid blocking group and control group (P<0.05).5. There was a significant difference between physiological saline group and mice liver homogenized solution group (P<0.05).6. There were no significant correlations between anti-egg nucleus antibody and IgG,IgM blood group antibodies.7. There was no significant difference on the anti-egg nucleus antibody level between mice fed with egg nucleus and mice fed with normal mice food (P>0.05).8. The level of anti-egg nucleus antibody decreased significantly in both of ICU acute disease group and ICU chronic disease group, comparing with control group respectively (P<0.05); There was statistical significant different level of IgM blood group antibody between control group and ICU acute diseases group (P<0.05); Significant different level of IgG blood group antibody was found between control group and ICU chronic diseases group (P<0.05).Conclusions:1. Anti-egg nucleus antibody was ubiquitous in random population.2. Reference limit of anti-egg nucleus antibody was not related with age or gender.3. The positive rate of HLA-DQB1*02 may be related to the high expression level of anti-egg nucleus antibody.4. Anti-egg nucleus antibody was not specific antibody to the egg nucleic acid ingredients. It possible combines with egg protein components.5. Anti-egg nucleus antibody did not show heterophile. 6. Anti-egg nucleus antibody was not related with the blood group antibodies. They independently exist in human body.7. The investigations of young mice which fed with egg nucleus didn't support that the antibody was food intolerant antibody which induced by food stimulation.8. Anti-egg nucleus antibody in critical patients significantly decree- sed. It can be used to evaluate the conditions of diseases.