The Effect Of Shenfu Injection On Myocardium Of Ultrastructure And Cell Injury During Periopration

Open heart surgery with cardiopulmonary bypass is very common operation in treating heart diease. But it lead to ischemia reperfusion injury, and have a lot of serious complication. The prevention of ischemia reperfusion injury is very important , which consists of reduction of surgical time and new techniques. Shenfu injection is from Chinese medicine, which has excellent ereat efficacity and less side-effects. Using shenfu injection during periopration to prevent ischemia reperfusion injury hasn't been reported. This experiment tries to supply the gap.Objective To investigate The effect of shenfu injection on myocardium of ultrastructure and cell injury during periopration.Methods 30 cases scheduled for cardiac surgery were randomly divided into tow group: test group(n=15)and control group(n=15). In the test group shenfu injection was injected by intrarenous drip for 1.5ml/kg·d. In the control group the treatment was common. Blood samples were withdrawn from coronary venous sinus before cross-damping and 10minutes,30minutes after aorta dedamping to measure the levels of the content of malondialdehyed (MDA),superoxide dismutase(SOD) and the serum cardinl troponin I(cTnI). Blood samples were collected from central vein before cross-clamping and 30minutes, 2hours, 24hours, 48hours after aorta declamping to detect creatine phosphokinase inoenzyme (CK-MB) an dlactate duhydrogenase(LDH). Myocardial ultrastructure was observed using electron microscope.Results MDA and cTnI levels in the test group were lower than those in the control group. 30minutes, 2hours, 24hours, 48hours after aorta declampingm, CK-MB and LDH were lower in the test group than the control group(P<0.05 or P<0.01). Elecrton microscope showed myocardium injury was lighted in test group than in control group. MAP was steady in test group, rather gradually increased in control group after aorta declamping. CVP and HR were steady in test group than that in control graup.Conclusion shenfu injection can protect myocardial mitochondria against ischemia-reperfusion injury in open heart operation with cardiopulmonary bypass.