| Broccoli, a cruciferous plant, is also called Brassica oleacea. The glucosinolates content of broccoli is very rich .Glucoraphanin is the main composition of glucosinolates(GSs) which is a secondary metabolites are higher bioactivity compounds found in special plants. The main biological activities of glucoraphanin is Sulforaphane which is due to hydrolysis products released by the action of myrosinase.Sulforaphane can be obtained form cruciferous vegetables as one of the products of enzymatic or acid hydrolysis of glucoraphanin, which has been indentified the best anticancer activity. Molecular weight is 177..29, formula is C6H11S2NO, structure as follows:In this paper, the optimum extraction technology getting high output of glucoraphanin from broccoli, the optimum glucoraphanin zymohydrolysis technology getting high output of sulforaphane from broccoli, the effect of sulforaphane and glucoraphanin on the immune system, effect of sulforaphane from broccoli on antitumor and preventing cancer, the stability of broccoli extract (27.9±1.2mg glucoraphanin/g) were studied, which established the groundwork for developing broccoli concentration Product.The experiment was carried out for finding out the optimum extraction technology getting high output of glucoraphanin from broccoli, the effects of solvent, varieties, parts, state, temperature, time on the extracting of glucoraphanin existed in broccoli were studied with broccoli as experiment material and the output of glucoraphanin as indicator. It was identified by HPLC. The optimized extraction processes were as follows: state: fresh broccoli; part: leave; solvent: 1:1, 95% A; extracting temperature is 70℃, extracting times is four。The experiment was carried out for finding out the optimum glucoraphanin zymohydrolysis technology getting high output of sulforaphane from broccoli, the effects of hydrolytic temperature, time on the extracting of sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] from glucoraphanin existed in broccoli were studied with broccoli as experiment material and the output of sulforaphane as indicator. It was identified by HPLC. The optimized extraction processes were as follows: fresh broccoli, hydrolytic temperature is 60℃, time is 6min; frozen broccoli: hydrolytic temperature is 55℃, time is 5min.The effect of sulforaphane and glucoraphanin on the immune system was studied by using BALB/c mice and the amount recommended by the human body was determining. Result: the immunomodulatory activity of glucoraphanin was indicated and the amount recommended by the human body (50kg) of glucoraphanin was 34 mg. The humoral immunomodulatory activity of sulforaphane was indicatedSulforaphane has been indentified the best anticancer activity by the experimental results of MTT. Significantly effect of broccoli extract (1.02mg sulforaphane /g) on tumor cell P338 was found with 200μg/mL.The experiment was carried out for accessing the effect of sulforaphane from broccoli on antitumor and preventing against cancer. Objective It is to observe the restraining tumor action of sulforaphane with different dosage on transplanted tumor H22 of mice and the influence on immune organ. Methods ICR species mice were chosen and randomly divided into five groups. The difference of restraining tumor rate of sulforaphane with different dosage only on transplanted tumor H22 of mice and the influence on thymus index, spleen index were observed. The animal test showed that the sulforaphane might have a little effect of antitumor of mice after mice (4∽6 weeks, body weight as 18∽22g) gavaged with 9g/kg dosage of broccoli extraction (1.02mg sulforaphane/g) daily for 8 days. Mice were used as animal experiments for accessing preventive cancer effect of sulforaphane. The mice were gavaged with the different dosage of broccoli extraction (maily active composition is sulforaphane) daily for 15 days. Then the nitric oxide (NO) content in serum and the glutathione S-transferase (GSTs) activities in liver, lung and kidney of mice were measured for accessing the effect of preventive cancer. The animal test showed that the NO content in serum of mice was reduced and GSTs activities was enhanced in liver, lung and kidney of mice with gavage dosage of sulforaphane increase. This indicated that the sulforaphane might have high effect of protecting against cancer of mice. Significantly effect of preventing against cancer was found after mice (12∽16 weeks, body weight as 30∽35g) gavaged with 1.85g/kg dosage of broccoli extraction (1.02mg sulforaphane /g) daily for 15 days. |
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